PXD062603

PXD062603 is an original dataset announced via ProteomeXchange.

Title	MdpS and MdpS2 structurally and functionally diverge to regulate Streptococcus oralis mucin degradation
Description	The human oral cavity harbors a diverse microbial community, with oral streptococci, particularly the Streptococcus mitis group, playing a pivotal role in biofilm formation and oral health. Among these, Streptococcus oralis is a key early colonizer that stabilizes oral biofilms. Here, we identify two mucin-degrading proteases, MdpS and MdpS2, that enable S. oralis to degrade MUC5B, the sole gel-forming mucin in saliva. Despite low sequence similarity, these enzymes share a high degree of tertiary structural resemblance and exhibits complementary biological functions. Their activity leads to extensive MUC5B degradation influencing biofilm dynamics by promoting biofilm dispersal and altering MUC5B and/or MUC5AC BCi mucus gels properties, with MdpS2 displaying specificity for MUC5B gels. Our findings reveal a specialized role in biofilm structural remodeling, offering potential avenues for clinical applications in biofilm modulation and mucus degradation.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:30:46.262.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liisa Arike
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-04 06:07:08	ID requested
⏵ 1	2025-11-09 16:30:47	announced

10.1080/20002297.2025.2571186;

Leo F, Nilsson J, Arike L, Kumar S, Hilton E, Lood R, Thornton DJ, Duncan GA, Svens, ä, ter G, Wickstr, ö, m C, . J Oral Microbiol, 17(1):2571186(2025) [pubmed]

submitter keyword: MUC5B,Streptococcus oralis, biofilm dynamics, mucin degrading proteases, Oral microbiome, mucin degradation

Fredrik Leo
contact affiliation	Department of Oral Biology and Pathology, Faculty of Odontology, Malmö University, Malmö, Sweden Genovis AB, Kävlinge, Sweden
contact email	fredrik.leo@mau.se
lab head
Liisa Arike
contact affiliation	PhD
contact email	liisaarike@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD062603

PRIDE project URI

• PRIDE
  1. PXD062603
     1. Label: PRIDE project
     2. Name: MdpS and MdpS2 structurally and functionally diverge to regulate Streptococcus oralis mucin degradation