PXD062593

PXD062593 is an original dataset announced via ProteomeXchange.

Title	Rational in vitro design of a two-phage cocktail against a contemporary Acinetobacter baumannii strain recovered from a burned patient at CHUV
Description	Acinetobacter baumannii is currently a major threat to human health. With the spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains, the development of complementary strategies is needed. A promising complimentary and realistic strategy could be phage therapy, which uses bacteriophages (phages), i.e viruses that specifically infect and kill bacterial cells during their life cycle. We designed a two-phage cocktail highly efficient against an extensive drug-resistant (XDR) A. baumannii isolate collected from a patient with burn wound infection at CHUV (termed Ab125). A first in vitro screen of our collection of 34 different phages identified only phage vB_AbaM_3098 as capable of lysing Ab125. However, quick selection of phage-resistant clones (termed Ab139) occurred. Comparative genomics and proteomics between Ab125 and Ab139 revealed several key variations. Very interestingly, we observed that Ab139 became susceptible to six different phages in the collection, otherwise inactive on Ab125. Phage-resistance was also selected when Ab139 was challenged with either of the six phages, with bacterial regrowth observed between 14 h and 16 h. However, combination of vB_AbaM_3098 and vB_AbaM_3014 led to a two-phage cocktail capable of totally inhibiting the growth of Ab125. Treatment with the phage cocktail led to 90% survival after 5 days in the in vivo Galleria Mellonella model of infectious diseases, compared to 0% in the non-treated group. We show that the combination of a phage that only slightly shifted the in vitro bacterial growth curve with an “inactive phage” led to the formulation of a highly bactericidal phage cocktail against Ab125. We then tested the therapeutic potential of the assembled cocktail in synergy with antibiotics and found a synergy with colistin. This work highlights the complexity sometimes involved in the assembly of potent phage cocktail.
HostingRepository	PRIDE
AnnounceDate	2025-12-08
AnnouncementXML	Submission_2025-12-07_16:18:07.557.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hugues de Villiers de la Noue
SpeciesList	scientific name: Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377); NCBI TaxID: NEWT:400667;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-04 02:06:07	ID requested
⏵ 1	2025-12-07 16:18:08	announced

de Villiers de la Noue H, Golliard G, Vuattoux X, Resch G, Strain Recovered from a Burned Patient at the Lausanne University Hospital. Viruses, 17(11):(2025) [pubmed]

10.3390/v17111441;

submitter keyword: A. baumannii, Phage cocktail, Phage therapy

Grégory Resch
contact affiliation	Laboratoire des bactériophages et de phagothérapie, CRISP, CHUV, Lausanne, Switzerland
contact email	gregory.resch@chuv.ch
lab head
Hugues de Villiers de la Noue
contact affiliation	laboratory of Bacteriophages and Phage Therapy, CRISP, CHUV
contact email	hugues.de-villiers-de-la-noue@chuv.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062593
     1. Label: PRIDE project
     2. Name: Rational in vitro design of a two-phage cocktail against a contemporary Acinetobacter baumannii strain recovered from a burned patient at CHUV