PXD062592

PXD062592 is an original dataset announced via ProteomeXchange.

Title	Thermal Proteome Analysis of nutrient stress in Cupriavidus necator expression dataset
Description	The facultative chemolithoautotroph Cupriavidus necator is capable of heterotrophic growth on diverse carbon sources or of autotrophic growth using CO2 fixation with H2 as an energy source. Under stress conditions, it is capable of producing biodegradable polyesters (polyhydroxyalkanoates, PHAs) as a storage material occupying a high proportion of the total biomass. This metabolic versatility means that C. necator is under intense study for sustainable biotechnology processes; however a relative lack of understanding of the overall regulatory architecture has limited its application. The major mechanisms by which proteins can respond to shifting cellular demands are protein expression change and/or allosteric regulation. Here we use two powerful proteomics methods to investigate these responses in C. necator cells grown on balanced and low nitrogen (PHA-inducing) media. Using quantitative proteomics and protein stability analysis (which can report on conformation change), we find that proteins across different pathways respond through one or both of these regulatory modes, including coordinated adaption to nutrient stress by the PHA pathway, the Calvin cycle, and ribosomal proteins.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:36:52.214.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062592
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eugene Dillon
SpeciesList	scientific name: Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha); NCBI TaxID: NEWT:381666; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-04 00:56:54	ID requested
⏵ 1	2025-11-09 16:36:53	announced

McKeever K, Tham JL, Bruch M, Narancic T, Connor KO, Upadhya SR, Ryan C, Dillon ET, Wynne K, Cagney G, Response to Nutrient Stress in the Industrial Model Bacterium Cupriavidus necator: A Thermal Proteome Profiling (TPP) Investigation. Proteomics, 25(20):25-38(2025) [pubmed]

10.6019/PXD062592;

10.1002/pmic.70045;

submitter keyword: Expression Proteomics,Thermal Proteomics

Gerard Cagney
contact affiliation	University College Dublin, School of Biomolecular and Biomedical Science, Conway Institute Belfield Dublin 4
contact email	gerard.cagney@ucd.ie
lab head
Eugene Dillon
contact affiliation	UCD
contact email	eugene.dillon@ucd.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062592
     1. Label: PRIDE project
     2. Name: Thermal Proteome Analysis of nutrient stress in Cupriavidus necator expression dataset