PXD062589
PXD062589 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Improving cellular fitness of human stem cell-derived islets under hypoxia |
Description | Stem cell-derived islet cell therapy can effectively treat type 1 diabetes, but its efficacy is hindered by low oxygen supply post-transplantation, particularly in subcutaneous spaces and encapsulation devices, leading to cell dysfunction. The response to hypoxia and effective strategies to alleviate its detrimental effects remain poorly understood. Here, we show that beta cells within stem cell-derived islets gradually undergo a decline in cell identity and metabolic function in hypoxia. This is linked to reduced expression of immediate early genes (EGR1, FOS, and JUN), which downregulates key beta cell transcription factors. We further identified genes important for maintaining beta cell fitness in hypoxia, with EDN3 as a potent player. Elevated EDN3 expression preserves beta cell identity and function in hypoxia by modulating genes involved in beta cell maturation, glucose sensing and regulation. These insights improve the understanding of hypoxias impact on stem cell-derived islets, offering a potential intervention for clinical applications. |
HostingRepository | MassIVE |
AnnounceDate | 2025-04-04 |
AnnouncementXML | Submission_2025-04-04_00:23:06.828.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Shlomi Brielle |
SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
ModificationList | TMTpro |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-04-03 23:59:33 | ID requested | |
⏵ 1 | 2025-04-04 00:23:07 | announced |
Publication List
no publication |
Keyword List
submitter keyword: hypoxia beta cells pancreas sc-islets, DatasetType:Proteomics |
Contact List
Doug Melton | |
---|---|
contact affiliation | Harvard University |
contact email | dmelton@harvard.edu |
lab head | |
Shlomi Brielle | |
contact affiliation | Harvard University |
contact email | shlomi.brielle@gmail.com |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097523/ |