PXD062562

PXD062562 is an original dataset announced via ProteomeXchange.

Title	Production of multifunctional hydrolysates from Lupinus mutabilis protein using a Micrococcus sp. PC7 protease
Description	The growing demand for functional foods has driven the search for bioactive com-pounds derived from plant proteins. Lupinus mutabilis “Tarwi,” a legume native to the Peruvian Andes, stands out for its high protein content and potential as a source of bi-oactive peptides (BPs). In this study, the functionality of the proteins contained in the albumin fraction (AF) isolated by tangential ultrafiltration (TFF) was investigated by using the OmicsBox software. The identified proteins were functionally classified into three groups: cellular components (35.57%), molecular function (33.45%), and biological process (30.97%). The isolated AF was hydrolyzed with the native protease PC7 (HAP), optimizing the E/S ratio and time parameters. Additionally, sequential hydrolysis of the PC7 protease and alcalase (HAPA) was performed. In vitro multifunctionality assays, HAP and HAPA demonstrated the ability to scavenge radicals (ABTS and ORAC), in-hibit angiotensin-converting enzyme (ACE)-I, and dipeptidyl peptidase IV (DPP-IV). The findings of this study highlight the potential of L. mutabilis albumin hydrolysate as a multifunctional ingredient for functional foods aimed at managing chronic conditions associated with oxidative stress, hypertension, and/or metabolic disorders.
HostingRepository	PRIDE
AnnounceDate	2025-06-02
AnnouncementXML	Submission_2025-06-01_16:07:20.015.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062562
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Samuel Paterson Moreno
SpeciesList	scientific name: Lupinus angustifolius; NCBI TaxID: 3871; scientific name: Lupinus albus; NCBI TaxID: 3870; scientific name: Phaseoleae; NCBI TaxID: 163735; scientific name: Lupinus mutabilis; NCBI TaxID: 53232;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-03 06:06:51	ID requested
⏵ 1	2025-06-01 16:07:20	announced

10.3390/biotech14020032;

10.6019/PXD062562;

Llontop-Bernab, é KS, Intiquilla A, Ramirez-Veliz C, Santos M, Jim, é, nez-Aliaga K, Zavaleta AI, Paterson S, Hern, á, ndez-Ledesma B, sp. PC7 Protease. BioTech (Basel), 14(2):(2025) [pubmed]

submitter keyword: Lupinus mutabilis

albumin fraction

ultrafiltration

Protease PC7

multifunctionality.

Blanca Hernández Ledesma
contact affiliation	Food Science Research Institue (CIAL, CSIC-UAM)
contact email	b.hernandez@csic.es
lab head
Samuel Paterson Moreno
contact affiliation	CIAL (CSIC-UAM)
contact email	samuel.paterson@csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD062562

PRIDE project URI

• PRIDE
  1. PXD062562
     1. Label: PRIDE project
     2. Name: Production of multifunctional hydrolysates from Lupinus mutabilis protein using a Micrococcus sp. PC7 protease