PXD062561

PXD062561 is an original dataset announced via ProteomeXchange.

Title	The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A
Description	The intrinsically disordered MYC proteins are master regulators of cellular growth and function, but when deregulated become cancer drivers. MYC-protein interactions are key to oncogenesis, and while disrupting such interactions would be of significant therapeutic benefit, the intrinsically disordered properties of MYC have dramatically hampered their characterization. Here, we apply an integrated structural biology approach to describe the structure and dynamics of the N-Myc–Aurora A complex, which is critical in neuroendocrine tumor progression. We reveal a functional interaction where multiple binding sites on N-Myc interact with the Aurora A N-lobe. The interaction is governed by aromatic clusters within the conserved MB0 and MBI motifs in N-Myc that interact with Aurora A in a dynamic binding mode that allosterically promotes kinase activation. We show that N-Myc binding to the Aurora A N-lobe can be inhibited by the small-molecule AurkinA, providing opportunity for therapeutical strategies to disrupt this interaction.
HostingRepository	PRIDE
AnnounceDate	2026-01-29
AnnouncementXML	Submission_2026-01-29_05:15:53.210.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simon Ekström
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-03 05:38:05	ID requested
⏵ 1	2026-01-29 05:15:53	announced

Dataset with its publication pending

submitter keyword: None

Maria Sunnerhagen
contact affiliation	Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
contact email	maria.sunnerhagen@liu.se
lab head
Simon Ekström
contact affiliation	BioMS - Swedish National Infrastructure for Biological Mass Spectrometry, Lund University, BMC D1330, 221 84 Lund, Sweden
contact email	simon.ekstrom@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD062561

PRIDE project URI

• PRIDE
  1. PXD062561
     1. Label: PRIDE project
     2. Name: The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A