PXD062559

PXD062559 is an original dataset announced via ProteomeXchange.

Title	Site-specific profiling of structure and function of IgM B cell receptor glycans
Description	Although N-linked glycans play pivotal roles in the regulation of antibody effector functions, little is known about the composition and functional impact of glycans expressed by human B cell receptors (BCRs), likely due to technical challenges. Here, we describe the site-specific glycosylation profiles of all four N-linked glycosylation-sites of human IgM BCRs from primary naive and memory B cells. We show that the BCR glycans have not undergone structural changes during the transition from naive to memory cells. Moreover, using B cell lines expressing well-defined BCRs, we show that individual glycosylation sites are non-essential for cell-surface expression, and that the absence of the IgM BCR N209 glycan reduces the antigen-binding capacity of B cells. Collectively, these findings shows high conservation of IgM BCR glycosylation across IgM BCR B cell subsets and indicate a limited contribution to BCR expression and function, suggesting BCR glycans may have evolved to support IgM antibody functions.
HostingRepository	PRIDE
AnnounceDate	2025-12-09
AnnouncementXML	Submission_2025-12-09_05:27:10.454.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rayman Tjokrodirijo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-03 04:56:03	ID requested
⏵ 1	2025-12-09 05:27:10	announced

Dataset with its publication pending

submitter keyword: IgM, Primary naïve and memory B cells, N-linked glycosylation,Human B cell receptors

Peter A. van Veelen
contact affiliation	Head Proteomics Group, Leiden University Medical Center, Center for Proteomics and Metabolomics, PO Box 9600, Postal zone P1-Q, 2300 RC Leiden, The Netherlands
contact email	P.A.van_Veelen@lumc.nl
lab head
Rayman Tjokrodirijo
contact affiliation	Leiden University Medical Center, Proteomics group
contact email	r.t.n.tjokrodirijo@lumc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062559
     1. Label: PRIDE project
     2. Name: Site-specific profiling of structure and function of IgM B cell receptor glycans