PXD062557

PXD062557 is an original dataset announced via ProteomeXchange.

Title	WAGO-4 Localization to Germ Granules Is Important for Its Accurate Small RNA Loading
Description	There were several MS experiments used in this study: 1) Proximity labeling of the P granules 2) Protein levels estimation from whole worm lysates and 3) wago-4 KO expression check (PRM analysis). 1) Proximity labeling strategy was used to estimate P granule composition in 3xFG mutant (FG domains of GLH-1, GLH-2, and GLH-4 mutated) and compare it to the WT. PGL-3 was tagged with TurboID at its native locus using CRISPR-Cas9. Biotinylation level was determined after streptavidin pull-down followed by MS analysis, serving as a proxy for protein enrichment in P granules. 2) Whole-worm lysate MS analysis was performed on 3xFG mutant worms to assess the worm proteome at an elevated temperature (26 °C), where these mutants exhibit a fecundity phenotype. 3) A STOP codon was introduced in the second exon of the WAGO-4 gene at its native locus in C. elegans (WAGO-4 KO). To assess whether the WAGO-4 protein is expressed in this strain, lysates from WAGO-4 KO (in triplicate) and N2 (WT control) were analyzed using PRM-MS.
HostingRepository	PRIDE
AnnounceDate	2025-10-14
AnnouncementXML	Submission_2025-10-14_10:17:02.387.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-03 03:44:33	ID requested
⏵ 1	2025-10-14 10:17:02	announced

10.1038/S44318-025-00606-X;

submitter keyword: C. elegans, proximity labeling, FG repeats, P granules

Stela Jelenic
contact affiliation	Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna
contact email	stela.jelenic@imba.oeaw.ac.at
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD062557

PRIDE project URI

• PRIDE
  1. PXD062557
     1. Label: PRIDE project
     2. Name: WAGO-4 Localization to Germ Granules Is Important for Its Accurate Small RNA Loading