PXD062545
PXD062545 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Age Shapes the Protein Corona: Unraveling the Impact of Biological Aging on Nanoparticle Identity and Function |
Description | Aging profoundly influences the composition of biological fluids, including the plasma proteome and lipidome, yet its effects on the protein coronas (PC) forming around nanoparticles (NPs) remain poorly understood. Here, we investigate how age-dependent changes in plasma proteins and lipids modulate the PC on silica NPs, affecting their physicochemical properties, cellular interactions, immune responses, and in vivo pharmacokinetics. Using plasma from C57 mice at 1, 9, and 18 months of age, we demonstrate that while lipid composition remains relatively stable, the PCs have subtle distinct age-related shifts. Proteomic analyses reveal altered abundances of proteins involved in endocytosis, cell adhesion, and immune regulation, ultimately influencing macrophage uptake and activation. In vitro experiments show that younger plasma-derived coronas enhance NP internalization, whereas older coronas diminish uptake but promote a stronger inflammatory response via complement activation and NF-κB signaling. In senescent macrophages, the pattern of NP internalization and immune activation differs, highlighting the complexity of age-associated changes in host-NP interactions. In vivo studies further confirm that NPs biodistribution, biocompatibility, and immunogenicity vary significantly with host age. our findings suggest age as a crucial yet underexplored factor in the design of nanomedicine, with significant implications for enhancing the safety and efficacy of both diagnostic and therapeutic nanomedicine technologies. |
HostingRepository | iProX |
AnnounceDate | 2025-04-02 |
AnnouncementXML | Submission_2025-04-02_21:03:42.800.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lirun Zhou |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | acetylated residue; methylthiolated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-04-02 21:02:55 | ID requested | |
⏵ 1 | 2025-04-02 21:03:43 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Si_Aged_PC |
Contact List
Jigang Wang | |
---|---|
contact affiliation | Artemisinin Research Center and Institute of Chinese Materia Medica, China Academy of Chinese Medical Science |
contact email | jgwang@icmm.ac.cn |
lab head | |
Lirun Zhou | |
contact affiliation | Artemisinin Research Center and Institute of Chinese Materia Medica, China Academy of Chinese Medical Science |
contact email | nzyzyb@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |