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PXD062521

PXD062521 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome data for Colorectal cancer cells transfected with different RNAs
DescriptionExon skipping (ES) events, known as the most prevalent form of alternative splicing, lead to the hallmark of tumorigenesis and considered to be therapeutic targets. To date, systematic functional interrogation of ES events in cancer are still largely unexplored. Here, we demonstrated the effectiveness of CRISPR-RfxCas13d in silencing transcripts derived from ES. Specifically, guide RNAs (gRNAs) targeting sequences spanning exon-skipping junction sites were designed and successfully employed without affecting their full-length counterparts. To comprehensively investigate ES events, we constructed a transcriptome-wide library of CRISPR-RfxCas13d gRNAs targeting 3,744 human ES events. We conducted loss-of-function screening both in vitro and in vivo using colorectal cancer (CRC) tumor cells. This approach led to the identification of several ES events crucial for cell growth. Notably, HMGN3 Δ6, a common oncogenic transcript derived from the ES of exon 6, exhibited a significant promotion of CRC proliferation. This work not only established CRISPR-RfxCas13d as a valuable tool for exploring and understanding ES events but also contributed to a comprehensive and large-scale characterization of the functional roles of ES events in CRC. The findings underscore the importance of ES events in CRC tumorigenesis, highlighting their potential as therapeutic targets.
HostingRepositoryPRIDE
AnnounceDate2025-06-12
AnnouncementXMLSubmission_2025-06-12_05:31:51.995.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterQiang Sun
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-04-02 05:46:02ID requested
12025-06-12 05:31:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Exon skipping, CRISPR-RfxCas13d, colorectal cancer, LC-MS/MS
Contact List
Qiang Sun
contact affiliationZhejiang University
contact emailsunqiangzai@zju.edu.cn
lab head
Qiang Sun
contact affiliationZhejiang University
contact emailsunqiangzai@zju.edu.cn
dataset submitter
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Dataset FTP location
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