PXD062511

PXD062511 is an original dataset announced via ProteomeXchange.

Title	Identification of p62-interacting proteins by mass spectrometry analysis in HEK293T cells
Description	HEK293T cells were ectopically expressing Flag tagged p62 (p62 group) or empty vectors (Control group) for 48h, cell lysates were incubated with anti-Flag affinity gels, and co-immunoprecipitates were subjected to trypsin digestion followed by mass spectrometry analysis. Peptides were separated by the EASY-nLC system (Thermo Fisher) and analyzed by the Q Exactive mass spectrometer (Thermo Fisher). Protein analysis were performed with Thermo Proteome Discoverer 2.1 (Thermo Fisher) and searched against Uniprot Human database. Three samples per group.
HostingRepository	PRIDE
AnnounceDate	2025-07-29
AnnouncementXML	Submission_2025-07-29_07:59:29.146.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yun yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-02 03:12:46	ID requested
⏵ 1	2025-07-29 07:59:29	announced

Qiu X, Ren J, Yang Y, Hu W, Wang C, Hu R, Li C, TRIM25 ubiquitinates and degrades p62/SQSTM1 to suppress autophagy. Acta Biochim Biophys Sin (Shanghai), ():(2025) [pubmed]

10.3724/abbs.2025062;

submitter keyword: HEK293T cells,p62, mass spectrometry analysis

yang yun
contact affiliation	The Second Affiliated Hospital Zhejiang University School of Medicine
contact email	yyang@ucas.ac.cn
lab head
yun yang
contact affiliation	Hangzhou Institute for Advanced Study
contact email	yyang@ucas.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062511
     1. Label: PRIDE project
     2. Name: Identification of p62-interacting proteins by mass spectrometry analysis in HEK293T cells