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PXD062503

PXD062503 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffects of hypoxia on lung cancer cells
Description300,000 of H358 cells were seeded in 6-well plates in quadruplicates per plate and placed either at 1 % or 21 % oxygen for either 48 or 72h, respectively. Upon the indicated time, cells were harvested in 500 µl of harvesting solution (80 % methanol in 50 mM ammonium acetate, supplemented with 2.5 mM N-ethylmaleimide (NEM) and heavy glutathione internal standard), sonicated for 5 s at 10 % amplitude and processed by for one-pot redox metabolite and protein thiol analysis approach. This project is in vitro validation of findings connected to the PRIDE project PXD052340.
HostingRepositoryPRIDE
AnnounceDate2025-05-12
AnnouncementXMLSubmission_2025-05-12_01:51:20.604.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTamara Tomin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListN-ethylmaleimide derivatized cysteine; monohydroxylated residue
InstrumenttimsTOF HT
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-04-02 00:13:50ID requested
12025-05-12 01:51:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: hypoxia, H358, timsTOF, lung cancer
Contact List
Ruth Birner-Gruenberger
contact affiliationUniv.Prof. PD DI Dr.techn. Professor for Bioanalytics TU Wien Faculty of Technical Chemistry Institute of Chemical Engineering and Analytics Department of Imaging and Analytical Chemistry Research Group Bioanalytics Getreidemarkt 9/E164 1060 Vienna Austria
contact emailruth.birner-gruenberger@tuwien.ac.at
lab head
Tamara Tomin
contact affiliationSenior Scientist at Institute of Chemical Technologies and Analytics Faculty of Technical Chemistry, TU Wien Getreidemarkt 9/164, 1060 Vienna
contact emailtamara.tomin@tuwien.ac.at
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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