PXD062503 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Effects of hypoxia on lung cancer cells |
Description | 300,000 of H358 cells were seeded in 6-well plates in quadruplicates per plate and placed either at 1 % or 21 % oxygen for either 48 or 72h, respectively. Upon the indicated time, cells were harvested in 500 µl of harvesting solution (80 % methanol in 50 mM ammonium acetate, supplemented with 2.5 mM N-ethylmaleimide (NEM) and heavy glutathione internal standard), sonicated for 5 s at 10 % amplitude and processed by for one-pot redox metabolite and protein thiol analysis approach. This project is in vitro validation of findings connected to the PRIDE project PXD052340. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-12 |
AnnouncementXML | Submission_2025-05-12_01:51:20.604.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tamara Tomin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | N-ethylmaleimide derivatized cysteine; monohydroxylated residue |
Instrument | timsTOF HT |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-04-02 00:13:50 | ID requested | |
⏵ 1 | 2025-05-12 01:51:20 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: hypoxia, H358, timsTOF, lung cancer |
Contact List
Ruth Birner-Gruenberger |
contact affiliation | Univ.Prof. PD DI Dr.techn. Professor for Bioanalytics TU Wien Faculty of Technical Chemistry Institute of Chemical Engineering and Analytics Department of Imaging and Analytical Chemistry Research Group Bioanalytics Getreidemarkt 9/E164 1060 Vienna Austria |
contact email | ruth.birner-gruenberger@tuwien.ac.at |
lab head | |
Tamara Tomin |
contact affiliation | Senior Scientist at Institute of Chemical Technologies and Analytics
Faculty of Technical Chemistry, TU Wien
Getreidemarkt 9/164, 1060 Vienna |
contact email | tamara.tomin@tuwien.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD062503 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062503
- Label: PRIDE project
- Name: Effects of hypoxia on lung cancer cells