PXD062503

PXD062503 is an original dataset announced via ProteomeXchange.

Title	Effects of hypoxia on lung cancer cells
Description	300,000 of H358 cells were seeded in 6-well plates in quadruplicates per plate and placed either at 1 % or 21 % oxygen for either 48 or 72h, respectively. Upon the indicated time, cells were harvested in 500 µl of harvesting solution (80 % methanol in 50 mM ammonium acetate, supplemented with 2.5 mM N-ethylmaleimide (NEM) and heavy glutathione internal standard), sonicated for 5 s at 10 % amplitude and processed by for one-pot redox metabolite and protein thiol analysis approach. This project is in vitro validation of findings connected to the PRIDE project PXD052340.
HostingRepository	PRIDE
AnnounceDate	2025-05-12
AnnouncementXML	Submission_2025-05-12_01:51:20.604.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamara Tomin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-ethylmaleimide derivatized cysteine; monohydroxylated residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-02 00:13:50	ID requested
⏵ 1	2025-05-12 01:51:20	announced

Dataset with its publication pending

submitter keyword: hypoxia, H358, timsTOF, lung cancer

Ruth Birner-Gruenberger
contact affiliation	Univ.Prof. PD DI Dr.techn. Professor for Bioanalytics TU Wien Faculty of Technical Chemistry Institute of Chemical Engineering and Analytics Department of Imaging and Analytical Chemistry Research Group Bioanalytics Getreidemarkt 9/E164 1060 Vienna Austria
contact email	ruth.birner-gruenberger@tuwien.ac.at
lab head
Tamara Tomin
contact affiliation	Senior Scientist at Institute of Chemical Technologies and Analytics Faculty of Technical Chemistry, TU Wien Getreidemarkt 9/164, 1060 Vienna
contact email	tamara.tomin@tuwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD062503

PRIDE project URI

• PRIDE
  1. PXD062503
     1. Label: PRIDE project
     2. Name: Effects of hypoxia on lung cancer cells