PXD062497
PXD062497 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome data of the Saccharopolyspora spinosa propionyl-CoA carboxylase engineered strain |
| Description | To enhance spinosad biosynthesis, we investigated the propionyl-CoA carboxylase (PCC) complex responsible for precursor synthesis. Through CRISPR interference (CRISPRi) and overexpression strategies, we constructed six engineered strains to systematically regulate the core PCC subunits encoded by pccA, pccB1, and pccB2. Upregulation of these genes enhanced PCC activity, improved energy and precursor utilization, and activated metabolic pathways, ultimately increasing spinosad production. |
| HostingRepository | iProX |
| AnnounceDate | 2025-04-02 |
| AnnouncementXML | Submission_2025-04-01_21:06:33.560.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ziyuan Xia |
| SpeciesList | scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-04-01 21:06:20 | ID requested | |
| ⏵ 1 | 2025-04-01 21:06:33 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Saccharopolyspora spinosa, propionyl-CoA carboxylase, DIA |
Contact List
| Liqiu Xia | |
|---|---|
| contact affiliation | Hunan Normal University |
| contact email | xialq@hunnu.edu.cn |
| lab head | |
| Ziyuan Xia | |
| contact affiliation | Hunan Normal University |
| contact email | 308437638@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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