PXD062489

PXD062489 is an original dataset announced via ProteomeXchange.

Title	Endogenous cathelicidin protects against Toxoplasma gondii- associated liver damage
Description	Toxoplasmosis, a disease caused by apicomplexan Toxoplasma gondii, is associated with various neuropsychiatric and behavioural conditions. Toxoplasmosis can cause serious complications for those with weakened immune systems and during pregnancy. Cathelicidins, peptides with antimicrobial and immunomodulatory functions, emerge as critical factors in host defence against microbes, but their role in parasitic infections is less understood. This study shows the function of endogenous cathelicidin protecting from the hepatic damage caused by T. gondii infection. Employing an oral infection model, we challenged wild-type (Camp+/+) and cathelicidin-deficient (Camp-/-) mice with low-virulent Type II strain of T. gondii (ME-49) cysts. Our findings demonstrate that Camp-/- mice exhibited exacerbated clinical manifestations, heightened mortality rates, and pronounced hepatic damage, and a lesser extent, splenitis relative to their Camp+/+ counterparts. Histological liver examinations indicated significant necrotic hepatitis in Camp-/- mice, correlating with increased local concentrations of pro-inflammatory cytokines and proteomic upregulation of PARP3 and GBP proteins. Increased cerebral inflammation and T. gondii cystogenesis were also observed in Camp-/- mice. Systemically, Camp-/- mice presented elevated levels of pro-inflammatory mediators, specifically interferon-gamma (Ifn-γ) and tumor necrosis factor-alpha (Tnf-α). In cultured macrophages, endogenous cathelicidin increased after T. gondii challenged while Camp-/- bone marrow derived macrophages released higher amounts of Tnf-α than their counterparts. We conclude cathelicidins confer protection against liver injury and systemic deterioration induced by T. gondii infection, lessening synthesis of pro-inflammatory cytokines.
HostingRepository	PRIDE
AnnounceDate	2026-02-19
AnnouncementXML	Submission_2026-02-19_13:28:55.015.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Young
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-01 16:42:06	ID requested
⏵ 1	2026-02-19 13:28:55	announced

Dataset with its publication pending

submitter keyword: cathelicidin

Toxoplasma gondii

liver

brain.

Antoine Dufour
contact affiliation	McCaig Insitute for Bone and Joint Health, Snyder Insitute for Chronic Diseases, Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada Department of Physiology & Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada
contact email	Antoine.dufour@ucalgary.ca
lab head
Daniel Young
contact affiliation	University of Calgary
contact email	daniel.young1@ucalgary.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062489
     1. Label: PRIDE project
     2. Name: Endogenous cathelicidin protects against Toxoplasma gondii- associated liver damage