PXD062473

PXD062473 is an original dataset announced via ProteomeXchange.

Title	Identification of NapM protein interactors in Mycobacterium smegmatis
Description	Nucleoid-associated proteins (NAPs) are key factors in maintaining chromosomal DNA integrity, gene regulation, DNA replication, and DNA repair. A recently identified mycobacterial NAP, NapMin (NapM) from Mycobacterium smegmatis, was a subject of the study. NapM modulates the expression of about one-third of M. smegmatis genes. Among these genes, those involved in cell envelope biosynthesis and cell division comprise a particular group specific to NapM. A bottom-up proteomics approach was utilized to define NapM interactors on protein level. For this purpose, a NapM pull-down was first performed from M. smegmatis cell lysate on antiFlagTag resin. Proteins associated with the resin were then identified via mass spectrometry. A WT cell lysate sample was parallelly processed in the exact same manner as background control.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-25_05:29:08.208.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD062473
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michał Tracz
SpeciesList	scientific name: Mycobacterium smegmatis; NCBI TaxID: NEWT:1772;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-01 10:21:14	ID requested
⏵ 1	2026-05-25 05:29:08	announced

10.6019/PXD062473;

submitter keyword: NapM PPI protein-protein interactions nucleoid associated protein mycobacterium smegmatis chrosome organization

dr Michał Tracz
contact affiliation	Laboratory of Protein Mass Spectrometry
contact email	michal.tracz2@uwr.edu.pl
lab head
Michał Tracz
contact affiliation	Laboratory of Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD062473

PRIDE project URI

• PRIDE
  1. PXD062473
     1. Label: PRIDE project
     2. Name: Identification of NapM protein interactors in Mycobacterium smegmatis