PXD062468

PXD062468 is an original dataset announced via ProteomeXchange.

Title	Tea(Camellia sinensis), Fresh tea leaves, Spring tea shoot, Bruker tims TOF Pro2 Mass spectrometer，
Description	In this study, a unique tea cultivar ‘Anxi kucha’ was discovered for the first time, which is rich in both secondary metabolites. In the targeted data, theacrine (17.44 mg/g) was detected only in the ‘Anxi kucha’ tea plant in the test materials. The content of EGCG3"Me in ‘Anxi kucha’ (11.25mg/g), ‘Tieguanyin’ (5.32mg/g) and ‘Fudingdabaicha’ (0.93mg/g) showed high, medium and low changes. Through proteomics and transcriptomics, it was identified that the key pathways for the synthesis of theacrine and EGCG3"Me were the purine metabolism pathway and the flavonoid biosynthesis pathway, respectively. Combined proteome-transcriptome-metabolome analysis showed that SAMS3, APRT1, IMPDH, and TCS1 were the main enzymes promoting theacrine synthesis; CHI1, CHI2, FLS2 and LAR1 were the main enzymes that promote the synthesis of EGCG3"Me. The results of transcription factor analysis showed that MYB4 and bHLH74 had positive regulatory effects on the synthesis of theacrine and EGCG3"Me. Theacrine and EGCG3"Me are both bitter compounds. This study provides a valuable material rich in theacrine and EGCG3"Me, which provides a material basis for further development and utilization in the field of tea health food and a theoretical basis for studying the functional components of tea.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:12:26.988.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanyu Zhu
SpeciesList	scientific name: Camellia sinensis; NCBI TaxID: 4442;
ModificationList	No PTMs are included in the dataset
Instrument	API 3000

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-01 09:10:26	ID requested
⏵ 1	2025-05-25 16:12:27	announced

10.1186/s12870-025-06691-8;

Zhu Y, Gu M, Yu W, Liao L, Gao S, Wang S, Lin H, Gui W, Zhou Y, Chen Z, Zeng J, Ye N, Multi-omics analysis of the mechanisms of abundant theacrine and EGCG3"Me in tea (Camellia sinensis). BMC Plant Biol, 25(1):663(2025) [pubmed]

ProteomeXchange project tag: Food and Nutrition (B/D-HPP)

submitter keyword: Proteomic Analysis of the 3rd Leaf Position of Spring Shoots Using DIA

Yanyu Zhu
contact affiliation	Fujian Agriculture and Forestry University
contact email	zyy8585065@163.com
lab head
Yanyu Zhu
contact affiliation	13569739371
contact email	zyy8585065@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD062468

PRIDE project URI

• PRIDE
  1. PXD062468
     1. Label: PRIDE project
     2. Name: Tea(Camellia sinensis), Fresh tea leaves, Spring tea shoot, Bruker tims TOF Pro2 Mass spectrometer，