PXD062450

PXD062450 is an original dataset announced via ProteomeXchange.

Title	CDKN1A stabilizes Cyclin D3 by inhibiting its phosphorylation-dependent nuclear export following butyrate treatment
Description	Butyrate-mediated inhibition of cell proliferation is part of the preventive role of dietary fiber against colorectal cancer (CRC). This effect notably involves the cyclin-dependent kinase inhibitor CDKN1A in human intestinal cells, yet the underlying molecular mechanisms remain incompletely understood. Previously, we observed a paradoxical increase in cyclin D3 (CCND3)—but not cyclin D1—levels upon butyrate exposure. Here, we demonstrate that the butyrate-induced accumulation of CCND3 results primarily from a CDKN1A-dependent stabilization, specifically extending its nuclear half-life. Proteomic analyses of CCND3 co-immunoprecipitates identified complexes involving CDKN1A, CDK4, CDK6, and the CRC-associated kinase CDK5, particularly enriched in butyrate-treated cells.
HostingRepository	PRIDE
AnnounceDate	2026-03-05
AnnouncementXML	Submission_2026-03-05_09:07:33.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carine Rodrigues
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-01 02:12:19	ID requested
1	2026-02-12 03:01:47	announced
2	2026-03-05 08:25:32	announced	2026-03-05: Updated project metadata.
⏵ 3	2026-03-05 09:07:33	announced	2026-03-05: Updated project metadata.

10.1186/S12964-026-02767-6;

submitter keyword: LC-MS/MS, butyrate, Homo sapiens, cyclin

Carine Machado Rodrigues
contact affiliation	Plateforme d'Analyse Proteomique Paris Sud-Ouest Université Paris-Saclay, INRAE, CNRS, AgroParisTech, Micalis Institute, 78352, Jouy en Josas, France
contact email	carine.rodrigues-machado@inrae.fr
lab head
Carine Rodrigues
contact affiliation	INRAe
contact email	carine.rodrigues-machado@inrae.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD062450

PRIDE project URI

• PRIDE
  1. PXD062450
     1. Label: PRIDE project
     2. Name: CDKN1A stabilizes Cyclin D3 by inhibiting its phosphorylation-dependent nuclear export following butyrate treatment