PXD062428 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing |
Description | Cell cycle progression is governed by complexes of the cyclin-dependent kinases (CDKs) and their regulatory subunits cyclin and Cks1. CDKs phosphorylate hundreds of substrates, often at multiple sites. Multisite phosphorylation depends on Cks1, which binds initial priming phosphorylation sites to promote secondary phosphorylation at other sites. Here, we describe a similar role for a recently discovered phosphate-binding pocket (PP) on B-type cyclins. Mutation of the PP in Clb2, the major mitotic cyclin of budding yeast, alters bud morphology and delays the onset of anaphase. Mutation of the PP reduces multi-site phosphorylation of CDK substrates in vitro, including the Cdc16 and Cdc27 subunits of the anaphase-promoting complex/cyclosome and the Bud6 and Spa2 subunits of the polarisome. We conclude that the cyclin PP, like Cks1, controls the pattern of multisite phosphorylation on CDK substrates, thereby helping to establish the robust timing of cell-cycle events. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-19 |
AnnouncementXML | Submission_2025-05-19_12:11:49.935.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD062428 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Robert Maxwell |
SpeciesList | scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-03-31 16:34:03 | ID requested | |
⏵ 1 | 2025-05-19 12:11:50 | announced | |
Publication List
Keyword List
submitter keyword: human, LFQ,CDK, phosphoproteomics,yeast,LCMS |
Contact List
David O. Morgan |
contact affiliation | David O. Morgan PhD Professor, Department of Physiology Vice Dean for Research, School of Medicine University of California, San Francisco Genentech Hall Room N312B 600 16th Street San Francisco CA 94143 415-476-6695 |
contact email | David.Morgan@ucsf.edu |
lab head | |
Robert Maxwell |
contact affiliation | UCB |
contact email | Robmaxwell@berkeley.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062428
- Label: PRIDE project
- Name: Phosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing