PXD062428

PXD062428 is an original dataset announced via ProteomeXchange.

Title	Phosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing
Description	Cell cycle progression is governed by complexes of the cyclin-dependent kinases (CDKs) and their regulatory subunits cyclin and Cks1. CDKs phosphorylate hundreds of substrates, often at multiple sites. Multisite phosphorylation depends on Cks1, which binds initial priming phosphorylation sites to promote secondary phosphorylation at other sites. Here, we describe a similar role for a recently discovered phosphate-binding pocket (PP) on B-type cyclins. Mutation of the PP in Clb2, the major mitotic cyclin of budding yeast, alters bud morphology and delays the onset of anaphase. Mutation of the PP reduces multi-site phosphorylation of CDK substrates in vitro, including the Cdc16 and Cdc27 subunits of the anaphase-promoting complex/cyclosome and the Bud6 and Spa2 subunits of the polarisome. We conclude that the cyclin PP, like Cks1, controls the pattern of multisite phosphorylation on CDK substrates, thereby helping to establish the robust timing of cell-cycle events.
HostingRepository	PRIDE
AnnounceDate	2025-05-19
AnnouncementXML	Submission_2025-05-19_12:11:49.935.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062428
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robert Maxwell
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-31 16:34:03	ID requested
⏵ 1	2025-05-19 12:11:50	announced

10.6019/PXD062428;

submitter keyword: human, LFQ,CDK, phosphoproteomics,yeast,LCMS

David O. Morgan
contact affiliation	David O. Morgan PhD Professor, Department of Physiology Vice Dean for Research, School of Medicine University of California, San Francisco Genentech Hall Room N312B 600 16th Street San Francisco CA 94143 415-476-6695
contact email	David.Morgan@ucsf.edu
lab head
Robert Maxwell
contact affiliation	UCB
contact email	Robmaxwell@berkeley.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD062428

PRIDE project URI

• PRIDE
  1. PXD062428
     1. Label: PRIDE project
     2. Name: Phosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing