⮝ Full datasets listing

PXD062428

PXD062428 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing
DescriptionCell cycle progression is governed by complexes of the cyclin-dependent kinases (CDKs) and their regulatory subunits cyclin and Cks1. CDKs phosphorylate hundreds of substrates, often at multiple sites. Multisite phosphorylation depends on Cks1, which binds initial priming phosphorylation sites to promote secondary phosphorylation at other sites. Here, we describe a similar role for a recently discovered phosphate-binding pocket (PP) on B-type cyclins. Mutation of the PP in Clb2, the major mitotic cyclin of budding yeast, alters bud morphology and delays the onset of anaphase. Mutation of the PP reduces multi-site phosphorylation of CDK substrates in vitro, including the Cdc16 and Cdc27 subunits of the anaphase-promoting complex/cyclosome and the Bud6 and Spa2 subunits of the polarisome. We conclude that the cyclin PP, like Cks1, controls the pattern of multisite phosphorylation on CDK substrates, thereby helping to establish the robust timing of cell-cycle events.
HostingRepositoryPRIDE
AnnounceDate2025-05-19
AnnouncementXMLSubmission_2025-05-19_12:11:49.935.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD062428
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRobert Maxwell
SpeciesList scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-03-31 16:34:03ID requested
12025-05-19 12:11:50announced
Publication List
10.6019/PXD062428;
Keyword List
submitter keyword: human, LFQ,CDK, phosphoproteomics,yeast,LCMS
Contact List
David O. Morgan
contact affiliationDavid O. Morgan PhD Professor, Department of Physiology Vice Dean for Research, School of Medicine University of California, San Francisco Genentech Hall Room N312B 600 16th Street San Francisco CA 94143 415-476-6695
contact emailDavid.Morgan@ucsf.edu
lab head
Robert Maxwell
contact affiliationUCB
contact emailRobmaxwell@berkeley.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD062428
PRIDE project URI
Repository Record List
[ + ]