PXD062414

PXD062414 is an original dataset announced via ProteomeXchange.

Title	Characteristics of tendinopathy development in humans
Description	Overuse injuries of tendon (tendinopathy) represents a significant musculoskeletal health problem, but its pathogenesis is only sparsely understood. We investigated the characteristics behind the development of tendinopathy in humans that had either early phase (1-2 months of symptoms, n=14) (ET) or more chronic (> 3 months, n=16) (CT) patella tendinopathy, vs healthy subjects (n=15) (CTRL). We found that pain symptoms rose and function declined with duration of injury, and that it was correlated to tendon size measured with MRI (3T and 7T). Tendon blood flow (US Doppler) increased gradually over the duration of tendinopathy, whereas peritendinous flow only rose in CT. Histological mapping (traditional multiplex and Cell Dive) of tendon vasculature showed marked changes only in CT, indicating that early in tendinopathy flow is increased in existing vessels whereas angiogenesis (both rise in vessel size and density) is a late phenomenon, and that tendinopathy leads to perivascular cell re-cruitment and expansion of lymphatic vessels. Further, matrisome analysis (proteomics) re-vealed that most matrix protein regulation occurs rather late in tendinopathy. It is concluded that the early phase of tendinopathy is dominated by pain symptoms that are correlated with tendon swelling together with increased blood flow due to flow regulation, whereas the more chronic state of tendinopathy is characterized by angiogenesis, and protein changes in the matrisome.
HostingRepository	PRIDE
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-01_23:53:59.236.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Marie Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-31 08:00:52	ID requested
⏵ 1	2026-03-01 23:54:00	announced

Merkel MFR, Malmgaard-Clausen NM, Lendal M, Siebner HR, Svensson RB, Dakin SG, Kr, ü, ger M, Schmidt L, Agergaard J, Yeung CC, Magnusson SP, Kjaer M, Increased Blood Flow and Tendon Swelling Precedes Vascular Expansion and Tissue Matrix Changes In Early Human Tendinopathy: A Potential Window for Superior Treatment Response. Adv Sci (Weinh), 13(12):e14023(2026) [pubmed]

10.1002/advs.202514023;

submitter keyword: ECM, clinical medicine,tendinopathy

Michael Kjaer
contact affiliation	1Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery, Copenhagen University Hospital – Bispebjerg and Frederiksberg; Copenhagen, Denmark 2Department of Clinical Medicine, University of Copenhagen; Denmark
contact email	michaelkjaer@sund.ku.dk
lab head
Luisa Marie Schmidt
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
contact email	luisa.schmidt@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD062414

PRIDE project URI

• PRIDE
  1. PXD062414
     1. Label: PRIDE project
     2. Name: Characteristics of tendinopathy development in humans