PXD062383

PXD062383 is an original dataset announced via ProteomeXchange.

Title	A Pseudomonas aeruginosa biliverdin IX-dependent transcriptional regulator (BdrB) integrates extracellular heme metabolism with Type IV pili-dependent motilities
Description	Pseudomonas aeruginosa displays remarkable adaptability in chronic infections by exploiting heme as its primary iron source at the expense of siderophore uptake. Previous studies have identified a pivotal role for the heme metabolites BVIX β/δ in the regulation of Type IV pili-dependent motility and biofilm formation. Thus, the heme metabolites integrate the switch in iron source with lifestyle adaptations required for chronic infection. The mechanism by which the BVIX metabolites directly or indirectly regulate such lifestyle adaptations remains elusive. In this study, through a combination of thermal proteome profiling (TPP) followed by quantitative mass spectrometry (MS), we identified a BVIX-binding protein PA5271 encoded in a three-gene operon (pa5269-pa-5270-pa5271). PA5271 is annotated as a helix-turn-helix DNA-binding protein and purifies as a dimer that binds BVIX but not BVIX or BVIX. Deletion of the gene renamed bdrB (biliverdin-dependent regulator responsive to BVIX) displayed a marked reduction in Type IV pili (TFP) associated motilities including twitching.
HostingRepository	PRIDE
AnnounceDate	2026-06-09
AnnouncementXML	Submission_2026-06-09_06:50:25.212.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mehari Weldemariam
SpeciesList	scientific name: Pseudomonas sp. AR40; NCBI TaxID: NEWT:1119017;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-30 16:25:49	ID requested
⏵ 1	2026-06-09 06:50:25	announced

Yue QX, Dai C, Kamatchinathan S, Bandla C, Webel H, Larrea A, Bittremieux W, Uszkoreit J, M, ü, ller TD, Xiao J, Cox J, Yu F, Ewels P, Demichev V, Kohlbacher O, Sachsenberg T, Bielow C, Bai M, Perez-Riverol Y, pmultiqc: An Open-Source, Lightweight, and Metadata-Oriented QC Reporting Library for MS Proteomics. Mol Cell Proteomics, 25(6):101530(2026) [pubmed]

10.1016/j.mcpro.2026.101530;

submitter keyword: biliverdin,Pseudomonas aeruginosa, heme metabolism, PA5271

Maureen A. Kane
contact affiliation	Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore
contact email	mkane@rx.umaryland.edu
lab head
Mehari Weldemariam
contact affiliation	University of Maryland Baltimore
contact email	mweldemariam@rx.umaryland.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062383
     1. Label: PRIDE project
     2. Name: A Pseudomonas aeruginosa biliverdin IX-dependent transcriptional regulator (BdrB) integrates extracellular heme metabolism with Type IV pili-dependent motilities