PXD062372

PXD062372 is an original dataset announced via ProteomeXchange.

Title	Unveiling the regulatory role of GRP7 in ABA signal-mediated mRNA translation efficiency regulation
Description	Abscisic acid (ABA) is a crucial phytohormone involved in plant growth and stress responses. While the transcriptional regulation triggered by ABA is well-documented, its effects on translational regulation have been less studied. Through Ribo-seq and RNA-seq analyses, we find that ABA treatment not only influences gene expression at the mRNA level but also significantly impacts mRNA translation efficiency (TE) in Arabidopsis thaliana. ABA inhibits global mRNA translation via its core signaling pathway, which includes ABA receptors, protein phosphatase 2Cs (PP2Cs), and SNF1-related protein kinase 2s (SnRK2s). Upon ABA treatment, Glycine-rich RNA-binding proteins 7 and 8 (GRP7&8) protein levels decrease due to both reduced mRNA level and decreased TE, which diminishes their association with polysomes and leads to a global decline in mRNA TE. The absence of GRP7&8 results in a global impairment of ABA-regulated translational changes, linking ABA signaling to GRP7-dependent modulation of mRNA translation. The regulation of GRP7 on TE relies significantly on its direct binding to target mRNAs. Moreover, mRNA translation efficiency under drought stress is partially dependent on the ABA-GRP7&8 pathways. Collectively, our study reveals GRP7’s role downstream of SnRK2s in mediating translation regulation in ABA signaling, offering a model for ABA-triggered multi-route regulation of environmental adaptation.
HostingRepository	PRIDE
AnnounceDate	2025-03-30
AnnouncementXML	Submission_2025-03-30_05:35:20.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Zhang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-29 06:18:52	ID requested
⏵ 1	2025-03-30 05:35:21	announced

10.1093/NAR/GKAE1011;

submitter keyword: ABA, translation efficiency, GRP7&8, Polysome profiling, Ribo-seq, drought

Jun Xiao
contact affiliation	Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
contact email	jxiao@genetics.ac.cn
lab head
Jing Zhang
contact affiliation	IGDB
contact email	jzhang@genetics.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD062372

PRIDE project URI

• PRIDE
  1. PXD062372
     1. Label: PRIDE project
     2. Name: Unveiling the regulatory role of GRP7 in ABA signal-mediated mRNA translation efficiency regulation