⮝ Full datasets listing

PXD062364

PXD062364 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Development and Clinical Evaluation of a Multiplexed Health Surveillance Panel Using Ultra High-Throughput PRM-MS in an Inflammatory Bowel Disease Cohort
Description	Despite advances in clinical proteomics, translating protein biomarker discoveries into clinical use remains challenging due to the technical complexity of the validation process. Targeted MS-based proteomics approaches like Parallel Reaction Monitoring offer sensitive and specific assays for biomarker translation. In this study, we developed a multiplex PRM assay using the combination of the Stellar-MS platform and the ART algorithm to quantify 57 plasma proteins, including 21 FDA-approved proteins. Loading curves (11-points) were conducted at 4 sample throughputs (100, 144, 180, and 300 samples per day) using independent, optimized, and scheduled PRM methods. Following optimization, an inflammatory bowel disease (IBD) cohort of plasma sample (496 IBD, 511 matched controls) was analyzed at a throughput of 180 SPD. To monitor system performance, the study also included 2,000 additional injections for system suitability tests, low, mid, and high-quality controls, washes, and blanks. Using this approach, we observed high quantifiability (linearity, sensitivity, reproducibility) in the PRM assay and consistency in data acquisition across a large cohort and validated the candidate IBD markers observed from discovery, particularly, CRP and A1AG1.
HostingRepository	PanoramaPublic
AnnounceDate	2025-09-21
AnnouncementXML	Submission_2025-09-21_17:05:01.906.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jihyeon Lee
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Stellar

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-28 16:46:44	ID requested
⏵ 1	2025-09-21 17:05:02	announced

Publication List

no publication

Keyword List

submitter keyword: Quantification, PRM, Stellar, Targeted Assay

Contact List

Jennifer Van Eyk
contact affiliation	Cedars Sinai Medical Center
contact email	Jennifer.VanEyk@cshs.org
lab head
Jihyeon Lee
contact affiliation	Cedars Sinai Medical Center
contact email	jihyeon.lee@cshs.org
dataset submitter

Full Dataset Link List

Panorama Public dataset URI