PXD062334

PXD062334 is an original dataset announced via ProteomeXchange.

Title	RNA binding protein hnRNPF deficiency drives malignant transformation
Description	1× 10e7 32D cells were lysed in 1 ml of hypotonic lysis buffer CEB-A (Applygen, Cat# 1200). Cells were disrupted by vortexing vigorously for 30 seconds, followed by incubation on ice for 15 minutes. During this period, samples were vortexed for 15 seconds every 5 minutes. Lysates were then centrifuged at 12,000g at 4°C for 5 minutes. The supernatant, containing the cytoplasmic protein fraction, was carefully transferred to a new tube without disturbing the pellet. The cytoplasmic lysates were incubated overnight at 4°C with either an HnRNPF antibody or IgG as a control. Protein G magnetic beads were added, and incubation continued for 2 hours at 4°C. After incubation, the beads were washed four times with wash buffer (50 mM Tris-HCl, 140 mM NaCl, 0.1% NP-40, 1 mM EDTA, 10% Glycerol), and the target proteins were eluted. The eluted proteins were resolved on a gradient gel and visualized by silver staining. Specific bands corresponding to both the control and experimental groups were excised and sent to Shanghai Applied Protein Technology (Shanghai, China) for mass spectrometry analysis to identify proteins interacting with HNRNPF in the cytoplasm.
HostingRepository	iProX
AnnounceDate	2025-03-28
AnnouncementXML	Submission_2026-03-31_02:06:46.576.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yapu Li
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-27 23:48:42	ID requested
⏵ 1	2026-03-31 02:06:47	announced

Dataset with its publication pending

submitter keyword: hnRNP F, HSC, AML, Lamp1, Translation

Tao Cheng
contact affiliation	Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China
contact email	chengtao@ihcams.ac.cn
lab head
Yapu Li
contact affiliation	Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	liyapu@ihcams.ac.cn
dataset submitter

iProX dataset URI