PXD062330

PXD062330 is an original dataset announced via ProteomeXchange.

Title	CAPTURE2.0 pull-down of SINE-MIR associated proteins in mouse embryonic stem cells
Description	In mouse embryonic stem cells, the regulators of transposable elements are not well known. Here, we studied the proteins regulating mammalian-wide interspersed repeat (MIR), a short-interspersed nuclear elements (SINE) family using the CAPTURE2.0 system. We generated cell lines expressing biotinylated dCas9 protein and MIR-targeting sgRNAs, performed biotin pulldown to isolate MIR-associated proteins, and analyzed them using mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-04-01
AnnouncementXML	Submission_2025-03-31_18:36:56.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yee Jiun Kok
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-27 20:26:35	ID requested
⏵ 1	2025-03-31 18:36:56	announced

Dataset with its publication pending

submitter keyword: Mouse, transposable elements, TMT, embryonic stem cells, LC-MS/MS

Xuezhi Bi
contact affiliation	Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01 Centros, Singapore 138668, Singapore
contact email	bi_xuezhi@bti.a-star.edu.sg
lab head
Yee Jiun Kok
contact affiliation	Bioprocessing Technology Institute
contact email	kok_yee_jiun@bti.a-star.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD062330

PRIDE project URI

• PRIDE
  1. PXD062330
     1. Label: PRIDE project
     2. Name: CAPTURE2.0 pull-down of SINE-MIR associated proteins in mouse embryonic stem cells