PXD062330 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CAPTURE2.0 pull-down of SINE-MIR associated proteins in mouse embryonic stem cells |
Description | In mouse embryonic stem cells, the regulators of transposable elements are not well known. Here, we studied the proteins regulating mammalian-wide interspersed repeat (MIR), a short-interspersed nuclear elements (SINE) family using the CAPTURE2.0 system. We generated cell lines expressing biotinylated dCas9 protein and MIR-targeting sgRNAs, performed biotin pulldown to isolate MIR-associated proteins, and analyzed them using mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-01 |
AnnouncementXML | Submission_2025-03-31_18:36:56.386.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yee Jiun Kok |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-03-27 20:26:35 | ID requested | |
⏵ 1 | 2025-03-31 18:36:56 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mouse, transposable elements, TMT, embryonic stem cells, LC-MS/MS |
Contact List
Xuezhi Bi |
contact affiliation | Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01 Centros, Singapore 138668, Singapore |
contact email | bi_xuezhi@bti.a-star.edu.sg |
lab head | |
Yee Jiun Kok |
contact affiliation | Bioprocessing Technology Institute |
contact email | kok_yee_jiun@bti.a-star.edu.sg |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062330
- Label: PRIDE project
- Name: CAPTURE2.0 pull-down of SINE-MIR associated proteins in mouse embryonic stem cells