PXD062326

PXD062326 is an original dataset announced via ProteomeXchange.

Title	Myosin Heavy Chain 10 dysregulation in infertile endometrial epithelial cells impairs adhesive capacity
Description	Endometrial receptivity is defined as the functional, morphological, and molecular changes of the endometrium occurring during a limited time period in the menstrual cycle called the ‘window of implantation’. The endometrial luminal epithelial cells are critical in producing this receptivity as their adhesive capacity enables embryo implantation; however, how this occurs is poorly understood. Recently, using endometrial epithelial organoids, we identified that Myosin Heavy Chain 10 (MYH10) was abnormally downregulated in infertile endometrial epithelial cells during the window of implantation, suggesting a role in receptivity. MYH10 regulates processes important in endometrial receptivity including cell polarity, adhesion, and migration; however, whether it regulates endometrial receptivity has yet to be researched. We aimed to investigate whether MYH10 regulates endometrial epithelial cell adhesive capacity. MYH10 localised to all major cellular compartments within endometrial tissue. However, immunostaining intensity was higher in luminal epithelial cells during the window of implantation compared to the proliferative phase in fertile endometrium. Conversely, this increased expression was not maintained in infertile endometrium during the window of implantation. siRNA knockdown of MYH10 in the receptive Ishikawa cell line significantly decreased cell adhesion to human cytotrophoblast-progenitor spheroids. MYH10 knockdown in Ishikawa cells also increased PGR, FOXO1, and GPX3 expression, while decreasing PDLIM2 expression. Proteomics analysis following MYH10 knockdown altered the production of 57 proteins with functions critical in endometrial receptivity including tight junction regulators. These results demonstrate that MYH10 alters endometrial epithelial cell adhesive capacity primarily via regulation of the actin cytoskeleton, implying an important role in implantation.
HostingRepository	PRIDE
AnnounceDate	2026-05-05
AnnouncementXML	Submission_2026-05-05_06:19:50.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michaela Sacco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-27 17:18:59	ID requested
⏵ 1	2026-05-05 06:19:51	announced

10.1098/rsob.250105;

Sacco M, Downing P, Santos LL, Varshney S, Teh WT, Zhou W, Dimitriadis E, Myosin heavy chain 10 dysregulation in infertile endometrial epithelial cells impairs adhesive capacity. Open Biol, 16(1):(2026) [pubmed]

submitter keyword: Endometrial Receptivity, Epithelium, Endometrial, Cell Adhesion, Actin Cytoskeleton,Infertility, MYH10, Myosin 2, Embryo Implantation Failure

Evdokia Dimitriadis
contact affiliation	Department of Obstetrics, Gynaecology and Newborn Health, The University of Melbourne, Parkville, Victoria, Australia. Gynaecology Research Centre, The Royal Women’s Hospital, Parkville, Victoria, Australia.
contact email	evdokia.dimitriadis@unimelb.edu.au
lab head
Michaela Sacco
contact affiliation	University of Melbourne
contact email	mssacc@student.unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD062326

PRIDE project URI

• PRIDE
  1. PXD062326
     1. Label: PRIDE project
     2. Name: Myosin Heavy Chain 10 dysregulation in infertile endometrial epithelial cells impairs adhesive capacity