PXD062303

PXD062303 is an original dataset announced via ProteomeXchange.

Title	Encephalomyocarditis virus protein 2B* antagonises innate immune signalling by interacting with 14-3-3 protein family members_C4PR_LIV
Description	Encephalomyocarditis virus (EMCV) has for decades served as an important model RNA virus. Although most of the EMCV proteins are obtained via proteolytic cleavage of a long polyprotein, 2B* is expressed from a short overlapping open reading frame via an unusual protein-stimulated temporally dependent ribosomal frameshifting mechanism. The function of 2B* has not yet been characterised, though mutant viruses that are unable to express 2B* have a small plaque phenotype. Here we show that 2B* binds all seven members of the 14-3-3 protein family during virus infection. Binding is dependent on the 2B* C-terminal sequence RRNSS. IFN-β and IL-6 signalling are impeded following overexpression of 2B* but not a truncated version lacking the RRNSS residues, thus suggesting a 14-3-3-dependent role for 2B* in inhibiting MAVS signalling. We also find that this function is distinct from the effect of 2B* on plaque size, as a virus in which 2B* was similarly truncated exhibited near-wildtype plaque size, thus indicating that 2B* also harbours additional functions. This work provides the first identification of a role of 2B* in innate immune antagonism and expands our knowledge of the protein complement of this important model virus.
HostingRepository	PRIDE
AnnounceDate	2025-08-12
AnnouncementXML	Submission_2025-08-12_10:04:54.346.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ed Emmott
SpeciesList	scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-27 03:40:10	ID requested
⏵ 1	2025-08-12 10:04:54	announced

Dataset with its publication pending

submitter keyword: AP-MS, TMT, 2B*,EMCV, Orbitrap

Edward Emmott
contact affiliation	University of Liverpool, Centre for Proteome Research, Biosciences Building, Crown Street, Liverpool, UK
contact email	e.emmott@liverpool.ac.uk
lab head
Ed Emmott
contact affiliation	Centre for Proteome Research, University of Liverpool
contact email	e.emmott@liverpool.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062303
     1. Label: PRIDE project
     2. Name: Encephalomyocarditis virus protein 2B* antagonises innate immune signalling by interacting with 14-3-3 protein family members_C4PR_LIV