PXD062298 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | TBK1 phosphorylates NAP1 on several sites |
| Description | NAP1 is known to be phosphorylated on several sites by TBK1. We decided to identify the phosphorylation sites on NAP1. For this, using an anti-GFP antibody, we immunoprecipitated a version of NAP1 fused to the GFP protein at its amino terminus (GFP-NAP1) from HEK293T cells co-expressing GFP-NAP1 and TBK1 fused to the mCherry protein at its amino terminus (mC-TBK1). Immunoprecipitates were analyzed by SDS-PAGE and WB, and the bands corresponding to NAP1 were cut and digested by trypsin protease before analysis by LC-MS/MS. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-16 |
| AnnouncementXML | Submission_2026-07-16_04:05:19.321.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD062298 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Laïla sago |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-27 02:02:38 | ID requested | |
| ⏵ 1 | 2026-07-16 04:05:19 | announced | |
Publication List
Keyword List
| submitter keyword: TBK1, NAP1, AZI2, phosphorylation |
Contact List
| Yves Gaudin |
|---|
| contact affiliation | Institute for Integrative Biology of the Cell (I2BC), CNRSUMR9198, 1 Avenue de la Terrasse F-91198 Gif-sur-Yvette CEDEX, France |
| contact email | Yves.gaudin@i2bc.paris-saclay.fr |
| lab head | |
| Laïla sago |
|---|
| contact affiliation | I2BC, CNRS |
| contact email | laila.sago@i2bc.paris-saclay.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062298
- Label: PRIDE project
- Name: TBK1 phosphorylates NAP1 on several sites
