PXD062288
PXD062288 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Candidate Arabidopsis MYB5-interacting proteins in developing siliques identified by IP-MS |
Description | 2 g developing siliques were harvested from the pMYB5:MYB5-GFP/myb5-2 complementary plants treated with 100 μM GA3 or 50 μM PAC. The 35S:GFP transgenic plants served as a negative control. Siliques were immediately crosslinked with 1% paraformaldehyde for 30 min on ice in a vacuum chamber. Total proteins were extracted using the Plant Cell lysis buffer for Western and IP (Beyotime, P0043) supplemented with phenylmethylsulfonyl fluoride (Solabio) and a proteinase inhibitor cocktail (Calbiochem). To reduce non-specific binding, protein extracts were pre-cleared with 50 μL of Binding control Agarose beads (ChromTek) for 1 h at 4 °C with gentle rotation. After brief centrifugation, the supernatant was incubated with 50 μL of GFP-Trap Agarose beads (ChromTek) for 2 h at 4 °C with gentle rotation. After 10 washes of the beads with phosphate-buffered saline, proteins on the beads were eluted through boiling in SDS-PAGE sample buffer for 10 min and then briefly separated in 10% SDS-PAGE gel. The gel regions containing proteins were cut and then subjected to LC-MS/MS analysis at Applied Protein Technology (Zhejiang). Three biological replicates were performed. The MS/MS database search was performed using the Maxquant software against the NCBI database with the default parameters (FDR < 0.01). |
HostingRepository | iProX |
AnnounceDate | 2025-03-27 |
AnnouncementXML | Submission_2025-05-19_00:19:21.022.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yan Xu |
SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker Daltonics amaZon series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-03-26 21:07:35 | ID requested | |
1 | 2025-03-26 21:07:49 | announced | |
⏵ 2 | 2025-05-19 00:19:21 | announced | 2025-05-19: Add Publication. |
Publication List
Xu Y, Du J, Hao R, Ma S, Ma Y, Zhou G, Hu R, Li S, Gibberellin signaling regulates pectin biosynthesis in Arabidopsis. Nat Commun, 16(1):4065(2025) [pubmed] |
Keyword List
submitter keyword: Arabidopsis, MYB5, IP-MS |
Contact List
Yan Xu | |
---|---|
contact affiliation | Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences |
contact email | xuyan@qibebt.ac.cn |
lab head | |
Yan Xu | |
contact affiliation | Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences |
contact email | xuyan@qibebt.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |