PXD062284

PXD062284 is an original dataset announced via ProteomeXchange.

Title	Metastatic Colorectal Cancer Cell Derived Extracellular Vesucles Aberrantly Activate Macrophages via Transfer of Neoplastic Ribosomes，eukaryotic initiation factors and tRNA ligases
Description	Exosomes play critical roles in colorectal cancer (CRC) in terms of oncogenesis, tumor cell survival, chemo-resistance, and metastasis. We have reported the proteome of exosomes released by human and mouse CRC cells. The proteins were predominantly focused on the translation regulation. We analyzed the exosomes of EMT CRC cell and epithelial-like CRC cell, and revealed that the upregulated proteins were predominantly enriched on the translation regulation. With a novel strategy, we traced the endpoint exosomally transported translation regulation proteins from CRC cells to macrophages and fibroblasts. Hence, we hypothesize that CRC cell derived exosomes regulate the globe translation of macrophage and fibroblasts, contributing to the formation of tumor microenvironment. We then employed our well-established model by using 3 CRC cell lines and macrophages to analyze the intracellular changes in terms of transcriptome and translatome, and characterized genome-wide translation regulation. The results showed that exosomes inhibited the translation process of fibroblasts due to the high ROS input from the tumor. We found that EMT CRC exosomes could up-regulate macrophage translation genome-wide, and promoted the transformation of macrophages to TAMs phenotype. we observed the preferential up-regulation of longer genes in macrophages treated with highly metastatic CRC exosomes, but not in those treated with Caco-2 exosomes. This study systematically researched the information flow of proteins from CRC cells to exosomes and then to target cells, and tempted to address the CRC cell exosomes educated macrophages in the systems biology view, which could lead to better understanding of translational control and its contribution to tumor microenvironment.
HostingRepository	iProX
AnnounceDate	2025-03-26
AnnouncementXML	Submission_2025-03-26_20:49:57.997.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingping Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	(13)C labeled residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-26 20:48:23	ID requested
⏵ 1	2025-03-26 20:49:58	announced

Dataset with its publication pending

submitter keyword: colorectal cancer, tumor microenvironment,exosome, proteome, translation regulation

Yang Wang
contact affiliation	Jinan University
contact email	wangyang0507@jnu.edu.cn
lab head
Qingping Chen
contact affiliation	Jinan University
contact email	cqp2023@stu2023.jnu.edu.cn
dataset submitter

iProX dataset URI