PXD062230

PXD062230 is an original dataset announced via ProteomeXchange.

Title	Unsupervised Multi-Omics Analysis Reveals Diagnostic Opportunities and Unseen Interactions in Equine Pituitary Pars Intermedia Dysfunction
Description	Properly integrating multiple modalities allows for a deeper understanding of complex biological processes. This study presents an unsupervised method of integrating proteomics, lipidomics, and metabolomics to reveal cross-omic trends, often overlooked by traditional analysis methods, in pituitary pars intermedia dysfunction (PPID). PPID is a neuroendocrine disorder in horses caused by the degeneration of hypothalamic dopaminergic neurons, leading to abnormal pituitary function. From the 6,436 detected molecular features, a traditional supervised approach found 201 significantly differentially expressed features. However, using Multi-Omics Factor Analysis (MOFA), trends not seen in the conventional approach were noted, such as divergence of C4BPA with triglycerides in the factor explaining the most lipidomic variance between the healthy and PPID-diagnosed horses, contradictory to current literary knowledge. Furthermore, using a random forest classifier, we highlighted potential biomarkers for PPID, which can be explored as potential diagnostic and therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2026-08-13
AnnouncementXML	Submission_2026-08-13_15:54:54.932.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maor Arad
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: NEWT:9796;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Ultra 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-25 16:40:12	ID requested
⏵ 1	2026-08-13 15:54:55	announced

Dataset with its publication pending

submitter keyword: Metabolomics, Unsupervised Analysis, Equus caballus, Multi-omics Factor Analysis (MOFA), Lipidomics, Equine Cushing’s Disease, Multi-Omics, Proteomics,Pituitary Pars Intermedia Dysfunction, LC-MS/MS

Leonard J. Foster
contact affiliation	Department of Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada. Proteomics and Metabolomics Core Facility, Life Sciences Institute, University of British Columbia, Vancouver, BC, V6T 2A1, Canada.
contact email	foster@msl.ubc.ca
lab head
Maor Arad
contact affiliation	University of British Columbia
contact email	maorarad1@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD062230

PRIDE project URI

• PRIDE
  1. PXD062230
     1. Label: PRIDE project
     2. Name: Unsupervised Multi-Omics Analysis Reveals Diagnostic Opportunities and Unseen Interactions in Equine Pituitary Pars Intermedia Dysfunction