PXD062224

PXD062224 is an original dataset announced via ProteomeXchange.

Title	Volatile Organic Compound and Proteomics Data from the Same Exhaled Breath Condensate Sample
Description	Obtaining multiple sample types from the same exhaled breath condensate (EBC) sample can reduce the number of samples needed for diagnostics purposes, allowing for sampling to be completed quicker and making it even easier to collect breath from patients. In this study, we performed analysis for volatile organic compounds (VOCs) and proteins from the same EBC sample. Pooled EBC samples were split into two groups: three samples that utilized immersion thin film-solid phase microextraction (TF-SPME) sampling for VOCs analysis and three samples that did not undergo TF-SPME sampling (non-TF-SPME). All six EBC samples were analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) for proteomics analysis. VOCs were analyzed via two-dimensional gas chromatography-mass spectrometry (GC x GC-MS). One hundred and eighty-four VOCs were found to be more abundant in EBC samples compared to blank or controls. There was no significant difference in the number of proteins detected in the TF-SPME samples compared to the non-TF-SPME samples and 144 of the 206 total unique proteins detected were found in both sample groups. These results indicate that TF-SPME sampling does not negatively affect the number of proteins that can be detected in EBC. This work is a step towards linking VOC and protein data together to obtain multi-omics breath data from a single breath sample.
HostingRepository	PRIDE
AnnounceDate	2025-07-20
AnnouncementXML	Submission_2025-07-19_17:14:38.268.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062224
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Shannon Schrader
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-25 13:43:24	ID requested
⏵ 1	2025-07-19 17:14:38	announced

10.6019/PXD062224;

submitter keyword: proteomics,exhaled breath condensate, liquid-chromatography-tandem mass spectrometry

Brooke L.
contact affiliation	Pacific Northwest National Laboratory
contact email	brooke.kaiser@pnnl.gov
lab head
Shannon Schrader
contact affiliation	Pacific Northwest National Laboratory
contact email	shannon.schrader@pnnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062224
     1. Label: PRIDE project
     2. Name: Volatile Organic Compound and Proteomics Data from the Same Exhaled Breath Condensate Sample