PXD062182

PXD062182 is an original dataset announced via ProteomeXchange.

Title	Sensory nerve-secreted factors regulate basal keratinocyte function in vitro
Description	Injuries to the skin can result in non-healing wounds, characterized by prolonged inflammation, failure to close, and chronic pain. Basal keratinocytes in the epidermis respond to signals activated following injury by proliferating, migrating, and differentiating to restore the epidermal barrier. The skin is densely innervated by peripheral sensory nerves, which contribute to the wound repair response. Although it is known that nerves are important for successful wound healing, the underlying cellular mechanisms of this phenomenon, and particularly the role of nerves in directing keratinocyte re-epithelialization, are poorly understood. To explore the relationship between sensory nerves and keratinocyte function in vitro, we cultured keratinocytes with conditioned media collected from dorsal root ganglia (DRG) in both homeostatic and post-wounding conditions and found that keratinocyte migration and proliferation, functions essential for re-epithelialization, were modulated by DRG conditioned media. Using a proteomic approach, we characterized the secretome of cultured DRG and identified key factors essential for wound healing, including extracellular matrix proteins, growth factors, and metabolic factors involved with ATP production, which was correlated with an increase in ATP rates of keratinocytes cultured in DRG conditioned medium. Our results advance our understanding of the microenvironmental cues that direct keratinocyte function during key events of cutaneous wound healing in vitro to drive the development of therapeutics that target dysregulated re-epithelialization in non-healing wounds.
HostingRepository	PRIDE
AnnounceDate	2025-03-28
AnnouncementXML	Submission_2025-03-28_11:07:10.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Avika Srivastava
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-24 14:43:14	ID requested
⏵ 1	2025-03-28 11:07:10	announced

10.1093/IOB/OBAF009;

submitter keyword: Wound healing, cell migration, cell proliferation, re-epithelialization, secretome

Samantha Louise Payne
contact affiliation	The University of Guelph, Guelph, Ontario, Canada. The department of Biomedical Sciences at the Ontario Veterinary College.
contact email	spayne@uoguelph.ca
lab head
Avika Srivastava
contact affiliation	The University of Guelph
contact email	avika@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD062182

PRIDE project URI

• PRIDE
  1. PXD062182
     1. Label: PRIDE project
     2. Name: Sensory nerve-secreted factors regulate basal keratinocyte function in vitro