PXD062171

PXD062171 is an original dataset announced via ProteomeXchange.

Title	Pull-down of polyP chains and associated proteins with PPBD as a bait
Description	CobB interacts polyP in E. coli stressed cells. The goal of the performed experiment was to identify proteins interacting polyP in E. coli cells encountering amino acid starvation. We used Co-NTA resin with immobilized PPBD for capturing and isolation of polyP, together with bound proteins. The MS analysis followed by a Mascot search against the E. coli database revealed 98 proteins identified (based on ≥ 3 peptides) in the extract prepared from wild-type E. coli MG1655 grown for 3 hours in MOPS. It must be pointed out that we obtained high background in the control lysate prepared from Δppk mutant, however in a comparative analysis, 21 proteins were qualified as interesting candidates that bind polyP in the tested conditions. Among them, CobB deacetylase was identified.
HostingRepository	PRIDE
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-01_16:07:24.729.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulina Czaplewska
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-24 08:25:47	ID requested
⏵ 1	2026-03-01 16:07:25	announced

10.1093/nar/gkag144;

Boguszewska E, Hirsz Z, Sroka M, Bury K, Chmura W, Strza, ł, ka A, Ko, ł, odziej M, Zakrzewska-Czerwi, ń, ska J, Konieczny I, PolyP inhibits CobB deacetylase triggering a regulatory coupling to prevent DNA replication initiation. Nucleic Acids Res, 54(4):(2026) [pubmed]

submitter keyword: polyP,E. coli MG1655 wild-type

Paulina Czaplewska
contact affiliation	University of Gdansk, Intercollegiate Faculty of Biotechnology, Proteomics Laboratory
contact email	Paulina.Czaplewska@ug.edu.pl
lab head
Paulina Czaplewska
contact affiliation	University of Gdansk
contact email	paulina.czaplewska@ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD062171

PRIDE project URI

• PRIDE
  1. PXD062171
     1. Label: PRIDE project
     2. Name: Pull-down of polyP chains and associated proteins with PPBD as a bait