PXD062162

PXD062162 is an original dataset announced via ProteomeXchange.

Title	Engineering low pH-tolerant Komagataella phaffii for industrial-ready itaconic acid production via methanol biorefinery
Description	This item is intended only as supplementary material for the study of the metabolic processes of the methanolic yeast _Komagataella phaffii for the production of itaconic acid.For the preparation of proteome samples, strains were cultured in Delft minimal medium using methanol as the sole carbon source. Cultivation was carried out at 30°C and 220 rpm until reaching mid-log phase (OD600 = 2). Cells were then collected by centrifugation at 12,000 rpm and 4 °C for 30 s and rapidly quenched in liquid nitrogen.Strains that do not produce itaconic acid were used as controls.
HostingRepository	iProX
AnnounceDate	2025-03-24
AnnouncementXML	Submission_2025-03-23_23:58:35.387.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fangqing Wei
SpeciesList	scientific name: Komagataella phaffii GS115; NCBI TaxID: 644223;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-23 23:58:18	ID requested
⏵ 1	2025-03-23 23:58:35	announced

Dataset with its publication pending

submitter keyword: Methanol biorefinery, Komagataella phaffii, Itaconic acid, Synthetic biology, Low pH fermentation

Ping Xu
contact affiliation	Shanghai Jiao Tong University
contact email	pingxu@sjtu.edu.cn
lab head
Fangqing Wei
contact affiliation	Shanghai Jiao Tong University
contact email	1511377317@qq.com
dataset submitter

iProX dataset URI