PXD062150

PXD062150 is an original dataset announced via ProteomeXchange.

Title	Comparison of N- and O-glycosylation on Spike Glycoprotein 1 of SARS-CoV-1 and MERS-CoV
Description	Coronaviruses (CoVs) are enveloped pathogens causing multiple respiratory disorders in humans with varying severity. Spike protein is one of the major proteins expressed on coronavirus surface, which mediates coronavirus entry into host cells. Spike proteins are extensively glycosylated and the glycans displayed on spike proteins play a key role in host pathogenesis and immune evasion. In this study, we aim to investigate whether glycosylation patterns are conservative at certain glycosites across different coronaviruses and how different host cells impact on the glycosylation profile. We analyzed site-specific glycans of S1 subunit from SARS-CoV and MERS-CoV spike proteins using hydrophilic interaction chromatography (HILIC) and LC-MS/MS on an Orbitrap Eclipse Tribrid mass spectrometer. We also compared glycosylation of MERS-CoV spike protein derived from HEK293 and insect cells. Our results show that SARS-CoV S1 and MERS-CoV S1 N-glycosylation presents some common patterns and also reveals the similar O-glycosites locations. Consistent with published data, confirming glycan and glycan subtype in specific positions, our data support that some monoclonal antibodies recognize glycan as part of their target epitope and cross react between SARS Cov and SARS CoV2 spike. The coronavirus spike proteins are highly glycosylated. The glycosylation sites, within each virus, are conserved with few changes over time.
HostingRepository	PRIDE
AnnounceDate	2025-04-07
AnnouncementXML	Submission_2025-04-07_13:38:16.750.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Bettinger
SpeciesList	scientific name: Severe acute respiratory syndrome coronavirus; NCBI TaxID: 2901879; scientific name: Middle East respiratory syndrome-related coronavirus; NCBI TaxID: 1335626;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-23 16:24:17	ID requested
⏵ 1	2025-04-07 13:38:17	announced

10.1021/ACS.JPROTEOME.4C00716;

submitter keyword: Glycosylation,SARS-CoV-1, MERS-CoV

John Cipollo
contact affiliation	FDA
contact email	John.Cipollo@fda.hhs.gov
lab head
John Bettinger
contact affiliation	FDA
contact email	john.bettinger@fda.hhs.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062150
     1. Label: PRIDE project
     2. Name: Comparison of N- and O-glycosylation on Spike Glycoprotein 1 of SARS-CoV-1 and MERS-CoV