PXD062081

PXD062081 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation profiling of phytocytokine versus MAMP signaling reveals systemin-specific signaling events
Description	Plasma membrane-localized pattern recognition receptors (PRRs) percieve host-derived phytocytokines (e.g. systemin) and microbe-associated molecular patterns (MAMPs; e.g. flg22 and chitin). Upon ligand binding, activation of PRRs induces similar early phosphorylation-regulated responses including extracellular alkalization and reactive oxygen species burst. However, despite similar early signaling events, the downstream responses are distinct: MAMPs trigger plant innate immunity, and the wound response is elicited by systemin. Therefore, the question arises as to how systemin-mediated responses differ from that of MAMPs, and how specificity is achieved. Here we used a Solanum peruvianum suspension cell culture to analyze phospho-proteomic responses triggered by 20nM flg22 and 15 ug/mL chitin compared to mock(water) treatment. Samples were analyzed in a time series of 0,1,2,5,15 and 45 min after elicitor treatment in six biological replicates. Sample numbering scheme:TreatmentTimeReplicate(e.g., F153 corresponds to flg22 treatment, 15min, the 3rd biological replicate; "C" and "H" are for chitin and water-treated samples,respectively.)
HostingRepository	PRIDE
AnnounceDate	2025-06-17
AnnouncementXML	Submission_2025-06-17_03:00:29.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rong Li
SpeciesList	scientific name: Solanum peruvianum; NCBI TaxID: 4082;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-20 08:14:27	ID requested
⏵ 1	2025-06-17 03:00:29	announced

10.1038/S41477-025-02040-7;

submitter keyword: phosphoproteomics,MAMP,time course,flg22,chitin

Andreas Schaller
contact affiliation	Department of Plant Physiology and Biochemistry, University of Hohenheim, 70599 Stuttgart, Germany
contact email	andreas.schaller@uni-hohenheim.de
lab head
Rong Li
contact affiliation	University of Hohenheim
contact email	rong.li@uni-hohenheim.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062081
     1. Label: PRIDE project
     2. Name: Phosphorylation profiling of phytocytokine versus MAMP signaling reveals systemin-specific signaling events