PXD062040
PXD062040 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | GNPS Proximity labeling identification of plasma membrane eisosome proteins in Candida albicans |
| Description | The ability of Candida albicans to resist stressful conditions in the host and grow invasively into tissues contributes to the virulence of this human fungal pathogen. Plasma membrane subdomains known as MCC (Membrane Compartment of Can1) or eisosomes are important for these processes. MCC/eisosome domains are furrow-shaped invaginations of the plasma membrane that are about 250 nm long and 50 nm deep. Two proteins that localize to these domains, Sur7 and Lsp1, are critical for virulence. Identifying additional proteins that localize to MCC/eisosomes has been hindered by the technical challenges of working with membrane proteins. Therefore, the proximity labeling method TurboID was used to identify MCC/eisosome proteins in C. albicans. Candidate proteins were identified by fusing a variant of BirA known as TurboID to either Sur7 or Lsp1, to promote biotinylation of nearby proteins. Analysis of 19 of these proteins by tagging with the Green Fluorescent Protein (GFP) identified 7 proteins that detectably overlapped with MCC/eisosomes. Deletion mutant analysis showed that one of these, a poorly studied protein known as Ker1, was important for hyphal growth in liquid culture, invasive growth into agar medium, and resistance to stress caused by copper and cell wall perturbing agents. Altogether, these approaches identified novel MCC/eisosome proteins and show that TurboID can be applied to better define the molecular mechanisms of C. albicans pathogenesis and aid in discovery of targets novel therapeutic strategies. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-04-23 |
| AnnouncementXML | Submission_2025-04-23_08:44:50.776.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | John Haley |
| SpeciesList | scientific name: Candida albicans; NCBI TaxID: 5476; |
| ModificationList | Phospho |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-19 10:51:19 | ID requested | |
| ⏵ 1 | 2025-04-23 08:44:51 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Candida albicans TurboID, DatasetType:Proteomics |
Contact List
| James B. Konopka | |
|---|---|
| contact affiliation | Stony Brook University |
| contact email | james.konopka@stonybrook.edu |
| lab head | |
| John Haley | |
| contact affiliation | Stony Brook University |
| contact email | john.haley@stonybrookmedicine.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097378/ |
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