⮝ Full datasets listing
PXD062019
PXD062019 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Global profiling of nascent chain interactors reveals TRIM25 as a novel co-translational E3 ubiquitin ligase |
| Description | Nascent polypeptide chains emerging from the ribosome engage a range of co-translational factors at distinct phases of translation. These co-translational interactions are crucial for proper protein biogenesis and quality control pathways to maintain protein homeostasis. Hence, systematic identification of these co-translational interactors provides insights into how distinct polypeptide fates are determined. Here, we developed Nascent Chain Interactor Profiling (NCIP), a metabolic labelling and chemical crosslinking enabled proteomics method to identify proteins interacting with nascent polypeptide chains at a proteome-wide scale. Results from NCIP support a widespread mechanism of co-translational assembly of protein complexes and reveal TRIM25 as a novel co-translational E3 ubiquitin ligase. TRIM25 ubiquitinates misfolded nascent polypeptide chains for quality control at the ribosome. Our results provide a generalisable framework to systematically profile nascent polypeptide chains interactors. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-03-19 |
| AnnouncementXML | Submission_2026-03-18_08:00:04.738.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Radoslaw Sobota |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; Met->Hpg; unknown modification |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-19 01:25:40 | ID requested | |
| ⏵ 1 | 2026-03-18 08:00:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: TMT, Proteomics, Translation, Interactome, Cross-linking |
Contact List
| Zhewang Lin | |
|---|---|
| lab head | |
| Radoslaw Sobota | |
| contact affiliation | IMCB A-STAR Singapore |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003714/ |
