PXD062012
PXD062012 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Epithelial-fibroblast crosstalk initiates protective mechanisms in epithelial cells upon acute injury |
| Description | Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal lung disease characterized by excessive fibrotic scar tissue accumulation, compromising lung function. While the precise etiology of IPF remains elusive, emerging evidence suggests that sustained lung epithelial injury is a key driver in the disease's development and progression. This persistent injury, unlike normal wound healing, maintains a pro-fibrotic niche that continually fuels the fibrotic process. Consequently, the question remains whether there is a way to alter this niche signaling, counteract the pro-fibrotic signals, and restore physiological wound healing. By approaching an epithelial-fibroblast coculture model, we investigated in the intercellular communication in chronic and acute injury situations. We identified that epithelial cells and fibroblasts together, create a protective niche signaling preventing further damage in acute injuries. Within epithelial cells, we observed that a switch of the metabolic state towards increased aerobic fatty acid metabolism initiates regenerative processes. While delineating secreted regulators responsible for inducing these positive responses, we identified pentraxin 3 (PTX3) as a top hit harboring antifibrotic characteristics. Modifying the niche by addition of PTX3 on chronically injured epithelial cells causes an amelioration of the pro-fibrotic phenotype and maintained the epithelial barrier integrity. These findings offer valuable insights in key differences of acute and chronic injuries and underscore the importance of understanding the complex interplay between epithelial cells and fibroblasts in lung injury and repair. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-07-20 |
| AnnouncementXML | Submission_2025-07-20_23:55:44.699.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alexandre Rosa Campos |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-18 22:08:01 | ID requested | |
| ⏵ 1 | 2025-07-20 23:55:45 | announced |
Publication List
| Marie-Therese Bammert, Ines Kollak, Jan Hoffmann, Eva Peter, Meshal Ansari, Holger Schlüter, Jun Li, Alexandre R. Campos, Coralie Viollet, Florian Gantner, Muriel Lizé, Matthew J. Thomas, Huy Q. Le. Dual roles of fibroblast-epithelial crosstalk in acute and chronic lung injury. Journal of Biological Chemistry (in press). |
Keyword List
| submitter keyword: Pulmonary fibrosis, Epithelial-fibroblast crosstalk, Fatty acid metabolism, PTX3, DatasetType:Proteomics |
Contact List
| Matthew J. Thomas | |
|---|---|
| contact affiliation | Boehringer Ingelheim Pharma GmbH |
| contact email | matthew_james.thomas@boehringer-ingelheim.com |
| lab head | |
| Alexandre Rosa Campos | |
| contact affiliation | SBP Medical Discovery Institute |
| contact email | arosacampos@sbpdiscovery.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097369/ |
to receive all new ProteomeXchange dataset release announcements!
