PXD061995

PXD061995 is an original dataset announced via ProteomeXchange.

Title	NEUTROPHILS SECRETE EXOSOME-ASSOCIATED DNA TO RESOLVE STERILE ACUTE INFLAMMATION
Description	Acute inflammation, characterized by a rapid influx of neutrophils, is a protective response that can lead to chronic inflammatory diseases when left unresolved. We previously showed that secretion of LTB4-containing exosomes via nuclear envelope (NE)-derived multivesicular bodies (MVBs) is required for effective neutrophil infiltration during inflammation. Here we report that the co-secretion of these exosomes with nuclear DNA facilitates the resolution of the neutrophil infiltrate in a mouse skin model of sterile inflammation. Activated neutrophils exhibit rapid and repetitive DNA secretion as they migrate directionally using a mechanism distinct from suicidal neutrophil extracellular trap (NET) release and cell death. Packaging of DNA in the lumen of NE-MVBs is mediated by lamin B receptor (LBR) and chromatin decondensation. These findings advance our understanding of neutrophil functions during inflammation and the physiological significance of DNA secretion.
HostingRepository	PRIDE
AnnounceDate	2025-06-04
AnnouncementXML	Submission_2025-06-03_16:24:51.355.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carole Parent
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-18 11:49:46	ID requested
⏵ 1	2025-06-03 16:24:51	announced

10.1038/S41556-025-01671-4;

submitter keyword: inflmmation, nuclear envelope, NETosis,Neutrophil, resolution, DNA secretion

Carole A.
contact affiliation	Life Sciences Institute, University of Michigan; Ann Arbor 48109, USA.
contact email	parentc@umich.edu
lab head
Carole Parent
contact affiliation	Life Sciences Institute, University of Michigan, Ann Arbor
contact email	parentclab@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD061995

PRIDE project URI

• PRIDE
  1. PXD061995
     1. Label: PRIDE project
     2. Name: NEUTROPHILS SECRETE EXOSOME-ASSOCIATED DNA TO RESOLVE STERILE ACUTE INFLAMMATION