PXD061991

PXD061991 is an original dataset announced via ProteomeXchange.

Title	Proteomics insights into alpaca embryo development
Description	South American camelids (SACs) play an important role in Andean economies, but reproductive inefficiencies, especially early embryonic losses, present significant challenges to herd productivity. This study provides the first proteomic analysis of preimplantation alpaca embryos, offering insights into the molecular mechanisms underlying embryo development and implantation. Using mass spectrometry, we identified 510 proteins in 15-day post-mating (dpm) alpaca embryos. Functional enrichment analysis revealed significant pathways related to cytoskeletal organization, metabolism, embryo elongation, and implantation. Key proteins associated with conceptus elongation, maternal recognition of pregnancy, and steroidogenesis were detected, including keratins (KRT8, KRT18), actin (ACTG1), annexin A2 (ANXA2), and enzymes involved in estradiol and cortisol biosynthesis. Additionally, proteins involved in prostaglandin synthesis, including PTGS2, were identified, supporting their role in conceptus elongation and implantation. This pioneering study provides a foundational dataset for understanding alpaca embryo development at the proteomic level, opening new paths for improving reproductive technologies in SACs. The findings highlight the molecular processes driving alpaca embryogenesis and provide a basis for future research on optimizing assisted reproductive technologies in camelids.
HostingRepository	PRIDE
AnnounceDate	2025-09-05
AnnouncementXML	Submission_2025-09-05_10:31:50.902.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ximena Castro
SpeciesList	scientific name: Vicugna pacos; NCBI TaxID: 30538;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-18 11:08:55	ID requested
⏵ 1	2025-09-05 10:31:51	announced

10.1016/J.THERIOGENOLOGY.2025.117529;

submitter keyword: Embryo,Alpaca, LC-MS/MS

Martín Eduardo Argañaraz
contact affiliation	Instituto Superior de InvestigacionesBiológicas (INSIBIO), CONICET‐UNT, and Instituto de Biología “Dr. FranciscoD. Barbieri,” Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, Argentina
contact email	martin.arganaraz@fbqf.unt.edu.ar
lab head
Ximena Castro
contact affiliation	INSIBIO-CONICET
contact email	xime.aixa.5@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD061991

PRIDE project URI

• PRIDE
  1. PXD061991
     1. Label: PRIDE project
     2. Name: Proteomics insights into alpaca embryo development