PXD061987

PXD061987 is an original dataset announced via ProteomeXchange.

Title	Protein composition of the biofilm matrix and the outer membrane vesicles (OMVs) during iron-sufficient and iron-restricted growth.
Description	There is limited efficacy in vaccines currently available to prevent some animal diseases of bacterial origin, such as bovine respiratory disease due to Histophilus somni. Protective efficacy can potentially be improved if bacterial antigens expressed in the host are included in vaccines. During H. somni infection in the bovine host, biofilms become established and the availability of essential iron is restricted. To investigate potential proteins as protective antigens, the protein composition of spontaneously released outer membrane vesicles (OMVs) during iron-sufficient and iron-restricted growth, and the proteins expressed in the biofilm matrix were analyzed and compared. Proteomic analysis revealed a dramatic physiological change in H. somni as it transitioned from the planktonic form to the biofilm mode of growth. All transferrin-binding proteins (Tbp) previously identified in H. somni were detected in the OMVs, suggesting that OMVs could induce antibodies to these proteins. Two TbpA-like proteins and 7 total proteins were present in the OMVs only when iron was restricted, indicating that the expression of these Tbp was differentially regulated. More proteins associated with quorum-sensing (QS) signaling were detected in the biofilm matrix compared with proteins in the OMVs, supporting a link between QS and biofilm formation. Proteins ACA31267.1 (OmpA) and ACA32419.1 (TonB-dependent receptor) were present in the OMV and biofilm matrix, and predicted to be potential protective antigens using an immuno-bioinformatic approach. Overall, the results support the development of novel vaccines that contain OMVs obtained from bacteria grown to simulate the in vivo environment, and possibly biofilm matrix, to prevent diseases due to bacterial pathogens.
HostingRepository	PRIDE
AnnounceDate	2025-05-15
AnnouncementXML	Submission_2025-05-15_12:50:32.312.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qin Fu
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-18 10:11:37	ID requested
⏵ 1	2025-05-15 12:50:32	announced

Dataset with its publication pending

submitter keyword: Histophilus somni, iron acquisition, outer membrane vesicles, protective antigen, reverse vaccinology, biofilm matrix

Qin Fu
contact affiliation	Cornell University
contact email	qf44@cornell.edu
lab head
Qin Fu
contact affiliation	Cornell University
contact email	qf44@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061987

PRIDE project URI

• PRIDE
  1. PXD061987
     1. Label: PRIDE project
     2. Name: Protein composition of the biofilm matrix and the outer membrane vesicles (OMVs) during iron-sufficient and iron-restricted growth.