PXD061976

PXD061976 is an original dataset announced via ProteomeXchange.

Title	The in vitro effect of omentin-1 on the global proteome of granulosa cells from normal weight Large White and fat Meishan pigs
Description	Granulosa cells (Gc) in the ovary play a vital role in nurturing oocyte development, producing estrogens, and maintaining the hormonal balance necessary for follicle maturation and successful ovulation. Omentin-1 (ITLN1) is an adipokine involved in energy metabolism and insulin resistance; its expression has been demonstrated in the ovary and varies depending on the degree of pig fatness. However, its effect on the global proteome of Gc has not been previously investigated. It was hypothesized that ITLN1 affects the abundance of proteins involved in key processes occurring in Gc during the mid-luteal phase of the estrous cycle in normal weight Large White and fat Meishan pigs, with potential variation based on the fat content of the pigs. Liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) analysis of porcine Gc identified 208 statistically significant differentially abundant proteins (DAPs) in the Large White pigs, with 99 proteins upregulated, and 109 downregulated. In Meishan pigs, 42 statistically significant DAPs were identified, including 25 upregulated and 17 downregulated proteins. Western blotting and ELISA analyses of six selected DAPs (cadherin 5, farnesyl diphosphate synthase, fibroblast growth factor receptor 1, matrix metallopeptidase 2, phosphatidylinositol-5-phosphate 4-kinase type 2 gamma, and yes-associated protein 1) confirmed the accuracy and precision of the LC‒MS/MS methodology. The identified DAPs were associated with several key biological processes, including the estrogen signaling pathway, cell cycle and DNA replication, protein synthesis, transport and maturation, cytoskeleton dynamics, cell signaling, and hormonal regulation. The results indicate that ITLN1 has a modulatory influence on the porcine Gc proteome, which is dependent on fat content. This highlights the important role of ITLN1 in regulating ovarian functions.
HostingRepository	PRIDE
AnnounceDate	2025-07-23
AnnouncementXML	Submission_2025-07-23_05:33:25.816.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061976
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Agata Malinowska
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-18 07:35:33	ID requested
⏵ 1	2025-07-23 05:33:26	announced

10.6019/PXD061976;

submitter keyword: LC‒MS/MS,omentin-1, proteome, pig, granulosa cells, Large White, Meishan

Agnieszka Rak
contact affiliation	Institute of Zoology and Biomedical Research Laboratory of Physiology and Toxicology of Reproduction, Faculty of Biology, Jagiellonian University, Krakow, Poland
contact email	agnieszka.rak@uj.edu.pl
lab head
Agata Malinowska
contact affiliation	IBB PAS
contact email	esme@ibb.waw.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061976
     1. Label: PRIDE project
     2. Name: The in vitro effect of omentin-1 on the global proteome of granulosa cells from normal weight Large White and fat Meishan pigs