PXD061975

PXD061975 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Pilocarpine inhibits Candida albicans biofilm maturation by altering lipid, sphingolipid, and protein content
Description	Candida albicans filamentation and biofilm formation are key virulence factors tied to tissue invasion and antifungal tolerance. Pilocarpine hydrochloride (PHCl), a muscarinic receptor agonist, inhibits biofilm maturation, though its mechanism remains unclear. We explored PHCl effects by analyzing sphingolipid and lipid composition and proteomics in treated biofilms. PHCl significantly decreased polar lipid and ergosterol levels in biofilms while inducing phytoceramide and glucosylceramide accumulation. PHCl also induced reactive oxygen species and early apoptosis. Proteomic analysis revealed that PHCl treatment downregulated proteins associated with metabolism, cell wall remodeling, and DNA repair in biofilms to levels comparable to those observed in planktonic cells. Consistently with ergosterol reduction, Erg2 was found reduced. Overall, PHCl disrupts key pathways essential for biofilm integrity, resulting in reduced stability and increased detachment, underscoring its potential as a versatile antifungal compound.
HostingRepository	MassIVE
AnnounceDate	2025-03-18
AnnouncementXML	Submission_2025-03-18_07:28:23.888.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	michele dei cas
SpeciesList	scientific name: Candida albicans; NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	instrument model

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-18 07:25:15	ID requested
⏵ 1	2025-03-18 07:28:24	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: untargeted lipidomics, DatasetType:Other (lipidomics)

submitter keyword: untargeted lipidomics, DatasetType:Other (lipidomics)

Contact List

Elisa Borghi
contact affiliation	Department of Health Sciences, Universita degli Studi di Milano, 20142 Milan, Italy
contact email	elisa.borghi@unimi.it
lab head
michele dei cas
contact affiliation	university of milan
contact email	michele.deicas@unimi.it
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v09/MSV000097355/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v09/MSV000097355/