PXD061926

PXD061926 is an original dataset announced via ProteomeXchange.

Title	Correlated cryogenic electron and mass imaging enables sub-cellular chemical mapping in bacteria
Description	Electron cryomicroscopy (cryo-EM) allows high-resolution spatial visualization of biological specimens, however, it is challenging to chemically identify densities observed in cryo-EM. To overcome this, we combined cryo-EM with chemical imaging using focused ion beam secondary ion mass spectrometry (FIB-SIMS) for integrated spatio-chemical analysis. We illustrate the correlative workflow using multiple examples, demonstrating sub-cellular localisation of molecules inside small bacterial cells. We used our workflow to illuminate the mechanism of uptake of Bisphenol-AF pollutant molecules by environmental bacteria, yielding insights into the storage of these chemicals within cytosolic phase-separated aggregates. Given the versatility of the workflow, which is compatible with cryogenic optical microscopy and FIB-milled lamellae of multicellular specimens, cryo-EM-FIB-SIMS is an effective avenue to map elemental and molecular signatures in cryo-EM.
HostingRepository	PRIDE
AnnounceDate	2026-05-13
AnnouncementXML	Submission_2026-05-13_08:18:07.095.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Catarina Franco
SpeciesList	scientific name: Caulobacter sp. S3H41; NCBI TaxID: NEWT:1190392;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-17 08:08:36	ID requested
⏵ 1	2026-05-13 08:18:07	announced

Dataset with its publication pending

submitter keyword: MS,CryoET

Tanmay A.M. Bharat
contact affiliation	Structural Studies Division MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge CB2 0QH, UK
contact email	tbharat@mrc-lmb.cam.ac.uk
lab head
Catarina Franco
contact affiliation	MRC Laboratory of Molecular Biology
contact email	cffranco@mrc-lmb.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD061926

PRIDE project URI

• PRIDE
  1. PXD061926
     1. Label: PRIDE project
     2. Name: Correlated cryogenic electron and mass imaging enables sub-cellular chemical mapping in bacteria