PXD061869

PXD061869 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling reveals ZFP36L1 as a central hub for post-transcriptional regulation networks in T cells
Description	Effective T cell responses against pathogens require a rapid yet tightly controlled remodeling of the proteome, and RNA binding proteins (RBPs) are key in this process. For instance, the RBP ZFP36L1 prevents excessive protein production and thereby limits immunopathology. ZFP36L1 is primarily known to mediate mRNA decay, but it can also regulate other processes. How its mode of action relates to its interaction partners is, however, not well-understood. Here, we mapped the ZFP36L1 interactome in primary human T cells. Using proximity labeling, we identified known and new interactors that regulate 3’UTR-mediated RNA degradation, deadenylation, stress granule/p-body formation, as well as 5’UTR-mediated translation repression and mRNA decapping. Snapshot analysis uncovered the ZFP36L1 interactome dynamics and RNA (in)dependency throughout T cell activation. Intriguingly, proximity labeling also uncovered regulators of ZFP36L1 protein expression: This included the helicase UPF1, which not only interacts with ZFP36L1 protein but also promotes its protein expression. Altogether, this comprehensive interactome map underlines the versatility of interactions with ZFP36L1 and their possible role in cellular function.
HostingRepository	PRIDE
AnnounceDate	2026-09-03
AnnouncementXML	Submission_2026-09-03_08:16:56.106.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arie Hoogendijk
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; timsTOF HT; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-14 17:01:18	ID requested
⏵ 1	2026-09-03 08:16:58	announced

10.1101/2025.07.25.666749;

submitter keyword: T cell,UltraID, RNA binding protein, post-transcriptional regulation

A.J. Hoogendijk
contact affiliation	Laboratory of mass spectrometry, Sanquin Research, Amsterdam, NL
contact email	a.hoogendijk@sanquin.nl
lab head
Arie Hoogendijk
contact affiliation	Sanquin Research, Amsterdam
contact email	a.hoogendijk@sanquin.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061869
     1. Label: PRIDE project
     2. Name: Proximity labeling reveals ZFP36L1 as a central hub for post-transcriptional regulation networks in T cells