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PXD061852

PXD061852 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEndogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
DescriptionThe human genome is replete with sequences derived from foreign elements including endogenous retrovirus-like proteins of unknown function. The human genome is replete with sequences derived from foreign elements including endogenous retrovirus-like proteins of unknown function. Here we show that UBQLN2, a ubiquitin-proteasome shuttle factor implicated in neurodegenerative diseases, is regulated by the linked actions of two retrovirus-like proteins, RTL8 and PEG10. RTL8 confers on UBQLN2 the ability to complex with and regulate PEG10. PEG10, a core component of stress granules, drives the recruitment of UBQLN2 to stress granules under various stress conditions, but can only do so when RTL8 is present. Changes in PEG10 levels further remodel the kinetics of stress granule disassembly and overall composition by incorporating select extracellular vesicle proteins. Within stress granules, PEG10 forms virus-like particles, underscoring the structural heterogeneity of this class of biomolecular condensates. Together, these results reveal an unexpected link between pathways of cellular proteostasis and endogenous retrovirus-like proteins. we show that UBQLN2, a ubiquitin-proteasome shuttle factor implicated in neurodegenerative diseases, is regulated by the linked actions of two retrovirus-like proteins, RTL8 and PEG10. RTL8 confers on UBQLN2 the ability to complex with and regulate PEG10. PEG10, a core component of stress granules, drives the recruitment of UBQLN2 to stress granules under various stress conditions, but can only do so when RTL8 is present. Changes in PEG10 levels further remodel the kinetics of stress granule disassembly and overall composition by incorporating select extracellular vesicle proteins. Within stress granules, PEG10 forms virus-like particles, underscoring the structural heterogeneity of this class of biomolecular condensates. Together, these results reveal an unexpected link between pathways of cellular proteostasis and endogenous retrovirus-like proteins.
HostingRepositoryPRIDE
AnnounceDate2025-06-04
AnnouncementXMLSubmission_2025-06-04_08:42:56.673.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLisa Sharkey
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion; Orbitrap Ascend
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-03-14 10:11:58ID requested
12025-06-04 08:42:57announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: stress granules, retrotransposon-derived proteins,Ubiquilins
Contact List
Lisa M. Sharkey
contact affiliationDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI 48109, United States
contact emaillisams@umich.edu
lab head
Lisa Sharkey
contact affiliationUniversity of Michigan
contact emaillisams@umich.edu
dataset submitter
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