PXD061842

PXD061842 is an original dataset announced via ProteomeXchange.

Title	Insights of the metabolic adaptation to low concentrations of ertapenem of a carbapenem resistant Klebsiella pneumoniae strain
Description	Klebsiella pneumoniae carrying antibiotic resistance against carbapenems are of great concern, due to the limited number of treatment options for infections caused by multiresistant bacteria. Novel antibiotics and treatment options are thus needed, and this need is exacerbated by the rapid and global spread of antibiotic resistances. In this study, we observe the global proteome changes of a K. pneumoniae strain (CCUG 70747) carrying carbapenem resistance genes under different concen-trations of ertapenem, a carbapenem antibiotic. Bottom-up tandem mass spectrometry (LC-MS/MS) was employed in combination with tandem mass tag (TMT) quantitative proteomics. Bioinformatics pipelines were used to observe changes in the GO terms and pathways associated with the differentially expressed proteins. The number of proteins detected with significant dif-ferential expression were 87 in the highest concentration applied and 61 in the lowest concentra-tion, all compared to the strain cultured without any antibiotics present. Several of these proteins, as well as the GO terms and pathways associated with the proteins, were linked to mechanisms of antibiotic resistance. However, this particular strain encodes potent β-lactamases, and thus, as expected, presented a reasonably modest impact in the global proteome upon exposure to the low concentrations of ertapenem applied.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_16:18:33.381.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Core Facility
SpeciesList	scientific name: Klebsiella pneumoniae; NCBI TaxID: NEWT:573;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-14 06:35:06	ID requested
⏵ 1	2025-10-05 16:18:34	announced

10.3390/ijms26188988;

Ja, é, n-Luchoro D, Salv, à, -Serra F, Pi, ñ, eiro-Iglesias B, Marathe N, Moore ERB, Karlsson R, Strain on Exposure to Sublethal Concentrations of Ertapenem. Int J Mol Sci, 26(18):(2025) [pubmed]

submitter keyword: Quantitative proteomics, Klebsiella pneumoniae, Mass spectrometry, Antibiotic resistance

Roger Karlsson
contact affiliation	Department of Infectious Diseases, Institute for Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden
contact email	roger.karlsson@gu.se
lab head
Proteomics Core Facility
contact affiliation	SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact email	gupcf@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD061842

PRIDE project URI

• PRIDE
  1. PXD061842
     1. Label: PRIDE project
     2. Name: Insights of the metabolic adaptation to low concentrations of ertapenem of a carbapenem resistant Klebsiella pneumoniae strain