PXD061829 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of intact proteomic profile of senescent-associated secretory phenotype (SASP) by top-down mass spectrometry. |
| Description | Senescent cells are increasingly recognized as major contributors to age-related diseases, primarily through the secretion of bioactive molecules known as the senescence-associated secretory phenotype (SASP). The SASP plays a crucial role in promoting various age-related conditions, including neurodegeneration and type-2 diabetes. While bottom-up proteomic profiling has been instrumental in elucidating senescence heterogeneity and linking SASP factors to aging and obesity, the complexity of protein modifications in SASP remains underexplored. Notably, proteoform-level analysis has not yet been applied to study SASP profiles in detail. In this study, we employ top-down proteomics to capture proteoform diversity in the secretomes of senescent and healthy lung fibroblasts (IMR90). |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-29 |
| AnnouncementXML | Submission_2025-12-28_16:16:53.873.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mozhgan Boroumand |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | dihydroxylated residue; sulfated residue; cysteinylation (disulfide with free L-cysteine); dehydrated residue; phosphorylated residue; myristoylated residue; acetylated residue; monohydroxylated residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-14 04:19:12 | ID requested | |
| ⏵ 1 | 2025-12-28 16:16:54 | announced | |
Publication List
| 10.1021/acs.analchem.5c02694; |
| Boroumand M, Dey A, Cupp-Sutton K, Pierce T, Tsitsipatis D, Herman AB, Wu S, Basisty N, Characterization of the Intact Proteomic Profile of Senescent-Associated Secretory Phenotype by Top-Down Mass Spectrometry. Anal Chem, 97(47):25967-25978(2025) [pubmed] |
Keyword List
| submitter keyword: senescence |
| aging |
| top-down proteomics |
| proteoforms |
| biomarkers |
| SASP |
| senescence-associated secretory phenotype |
Contact List
| Nathan Bassity |
|---|
| contact affiliation | National Institut on Aging, NIH, Baltimore MD, USA |
| contact email | nathan.basisty@nih.gov |
| lab head | |
| Mozhgan Boroumand |
|---|
| contact affiliation | National Institute on Aging, NIH, Baltimore MD 21224 |
| contact email | mojgan.boroumand@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061829
- Label: PRIDE project
- Name: Characterization of intact proteomic profile of senescent-associated secretory phenotype (SASP) by top-down mass spectrometry.
