PXD061805
PXD061805 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In situ nuclear pore complex of the higher plant Arabidopsis thaliana |
| Description | The nucleus is delimited by the nuclear envelope (NE), where nuclear pore complexes (NPCs) are embedded. While this complex has been well studied in vertebrates, yeast, and, more recently, in algae, in situ structural data of higher plants is still missing. Here, we show that many individual nucleoporins of Arabidopsis thaliana and human present high structural similarity. We report a first higher plant in situ NPC structure, derived from A. thaliana root protoplasts using cryo electron tomography, subtomogram averaging and homology-based integrative modeling. We present a plant NPC model based on predicted models of A. thaliana NUPs identified by mass spectrometry. The plant NPC scaffold exhibits differences in diameter and height to C. reinhardtii, while sharing some structural features with H. sapiens NPCs. Notably, we observed that the A. thaliana NPC contains NUP155 connectors like the H. sapiens NPC, which may explain the height difference when compared to the C. reinhardtii NPC. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-09-05 |
| AnnouncementXML | Submission_2025-09-05_05:54:14.276.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Victor Fourcassie |
| SpeciesList | scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-13 11:54:36 | ID requested | |
| ⏵ 1 | 2025-09-05 05:54:14 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Arabidopsis thaliana, Nuclear pore complex (NPC), NUPs, DatasetType:Proteomics |
Contact List
| Hugo Germain | |
|---|---|
| contact affiliation | Universite du Quebec a Trois Rivieres |
| contact email | germainhugo@gmail.com |
| lab head | |
| Victor Fourcassie | |
| contact affiliation | CRCHU Proteomics platform |
| contact email | victor.fourcassie@crchudequebec.ulaval.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097314/ |
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